Curcuma longa L. is widely used as spices and medicines. Leaves, stems and rhizomes of the plant contain essential oils and provide a useful habitat for different endophytic fungi groups. Strain of Aspergillus terreus was isolated from Curcuma longa L. and was determined to be capable of producing secondary metabolites against bacteria by disc diffusion method and optimization of culture conditions by Design-Expert 6.0.6 and BC Pharsoft software. This research identified of the culture conditions for A. terreus N-GL1 strain produced secondary metabolites against Staphylococcus aureus and MRSA (Methicillin resistant Staphylococcus aureus) good: appropriate carbon source was saccharose 1% or molasses 2,2%; suitable nitrogen sources are potatoes 10% at pH 7; initial amount of yeast cells was 104 CFU/ml; incubated at room temperature for seven day. This study was found out as suitable conditions for A. terreus N-GL1 strain produced the secondary metabolites against S. aureus and MRSA.
The study has isolated endogenous A. terreus-RTN3 strain from young stems of Alpinia chinensis Rosc.-Zingiberaceae family. Cultivating and extracting compounds containing metabolites that are resistant to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA). Determining the number of antibacterial compounds in the crude extract by thin-layer chromatography and vacuum liquid chromatography (VLC), the research has isolated pure MM2 compound with high antioxidant activity and spectral resolution to find the structure compound MM2 (Methyl 2-((4-amino-2-bromo-3-methyl-5-thioxocyclopenta-1,3-dien-1-yl) oxygen) -4-hydroxy-6-methoxybenzoate). This is a new compound that has not been announced in any works in the world. MM2 compound has a high antioxidant activity, the ability to catch α, α-diphenyl-β-picrylhydrazyl (DPPH) free radicals increases linearly by concentration, at concentration 400ppm of MM2 is capable of catching over 75% of DPPH free radicals. The MM2 compound also has the ability to inhibit 4 test cell lines: breast cancer (MCF-7), cervical cancer (Hela), liver cancer (Hep G2) and lung cancer (NCI-H460). The results showed that MM2 compound’s ability to inhibit cancer cell lines increased linearly, at concentrations of 100 µg/ml of MM2 compound, which inhibited nearly 80% of cancer cell lines and was high most in liver cancer cell line HepG2 (at a concentration of 100 µg/ml inhibits 86.82% of liver cancer cells HepG2).
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