Determination of structure and anticancer activity of MM₂ compound, isolated from endophytic fungus Aspergillus terreus-RTN3 of Alpinia chinensis Rosc.

Abstract

The study has isolated endogenous A. terreus-RTN3 strain from young stems of Alpinia chinensis Rosc.-Zingiberaceae family. Cultivating and extracting compounds containing metabolites that are resistant to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA). Determining the number of antibacterial compounds in the crude extract by thin-layer chromatography and vacuum liquid chromatography (VLC), the research has isolated pure MM2 compound with high antioxidant activity and spectral resolution to find the structure compound MM2 (Methyl 2-((4-amino-2-bromo-3-methyl-5-thioxocyclopenta-1,3-dien-1-yl) oxygen) -4-hydroxy-6-methoxybenzoate). This is a new compound that has not been announced in any works in the world. MM2 compound has a high antioxidant activity, the ability to catch α, α-diphenyl-β-picrylhydrazyl (DPPH) free radicals increases linearly by concentration, at concentration 400ppm of MM2 is capable of catching over 75% of DPPH free radicals. The MM2 compound also has the ability to inhibit 4 test cell lines: breast cancer (MCF-7), cervical cancer (Hela), liver cancer (Hep G2) and lung cancer (NCI-H460). The results showed that MM2 compound’s ability to inhibit cancer cell lines increased linearly, at concentrations of 100 µg/ml of MM2 compound, which inhibited nearly 80% of cancer cell lines and was high most in liver cancer cell line HepG2 (at a concentration of 100 µg/ml inhibits 86.82% of liver cancer cells HepG2).