Reports indicate an increase in the incidence of DNA fragmentation in male factor infertility and its role in the outcome of assisted reproductive techniques (ART). However, reports are conflicting between the relationships of sperm DNA integrity with conventional semen parameters. We examined the relationship between conventional sperm parameters and DNA integrity using acridine orange (AO) test. The study included 373 patients and 28 fertile volunteers. DNA normality was compared with semen parameters between the patient and donor populations. Significant correlations were noted between DNA normality and sperm concentration (r = 0.18, P = 0.000), motility (r = 0.21, P = 0.0001), rapid motility (0.19, P = 0.000), normal morphology by World Health Organization (r = 0.15, P = 0.019) and head defects (r = -0.15, P = 0.023). A significant difference was noted in AO levels between donors and patients with asthenozoospermia (P = 0.002) and oligoasthenozoospermia (P = 0.001). A significant difference in DNA integrity was noted in samples having <30% and >30% normal morphology. A wide range of % DNA normality was observed in the patient group. Sperm assessment for DNA status using AO is reliable and shows good correlation with sperm count, motility and morphology. Assessment of sperm DNA status with AO staining may be helpful prior to ART.
The Eastern Ghats Belt is a polycyclic granulite terrain along the east coast of India whose western boundary is marked by a shear zone along which the granulites are thrusted over the cratonic units of the Indian shield, and its northern margin is marked by the presence of a number of fault-bounded blocks. Recent work has convincingly brought out that there are domains within the belt having different evolutionary histories. The segment south of the Godavari Rift went through a high grade thermo-tectonic event at ~1.6-1.7 Ga. North of the Godavari Rift in a narrow zone along the western boundary the last high-grade metamorphic event is of late Archaean age. A series of alkaline plutons along the western boundary zone testifies to a rifting episode at ~1.3-1.5 Ga. In the major part of the EGB the metamorphism is broadly of Grenvillian age, with two major thermo-tectonic pulses at ~1.1-1.2 Ga and ~0.95-1.0 Ga. But high grade conditions persisted for a long period and younger thermal events of ~0.65 Ga to ~0.80 Ga are locally recorded. There are differences in the tectonometamorphic histories of different domains, but the tectonic significance of these differences remains uncertain. Pan-African (0.50-0.55) thermal overprints are common and become conspicuous along the western boundary zone. The thrusting of the Eastern Ghats granulites in a hot state over the cratons to the west is of Pan-African age. In the Rodinia assembly (~0.9 Ga) the Eastern Ghats and the Rayner-Napier Complexes of Antarctica were contiguous, but the pre-Rodinia configuration of these terrains remains unclear. At ~0.8 Ga during the Rodinia break up Greater India rifted apart from East Antarctica, and only later it docked with Australia-East Antarctica at 530-550 Ma. The continuation of the East Antarctic Pan-African orogenic belts into the Eastern Ghats is yet to be ascertained.
Arsenic contamination in groundwater is becoming more and more a worldwide problem. Nearing 50 million of people are at health risk from arsenic contamination at Ganga-Meghna-Bramhaputra basin. The experimental results of the five blocks under Malda district of West Bengal, India, showed that the arsenic concentration in groundwater (0.41-1.01 mg/l) was higher than the permissible limit for drinking water (0.01 mg/l) (WHO) and FAO (Food and Agriculture Organization) permissible limit for irrigation water (0.10 mg/l). The soil arsenic level (13.12 mg/kg) crossed the global average (10.0 mg/kg), but within the maximum acceptable limit for agricultural soil (20.0 mg/kg) recommended by the European Union. The total arsenic concentration on food crops varied from 0.000 to 1.464 mg/kg of dry weight. The highest mean arsenic concentration was found in potato (0.456 mg/kg), followed by rice grain (0.429 mg/kg). The total mean arsenic content (milligrams per kg dry weight) in cereals ranged from 0.121 to 0.429 mg/kg, in pulses and oilseeds ranged from 0.076 to 0.168 mg/kg, in tuber crops ranged from 0.243 to 0.456 mg/kg, in spices ranged from 0.031 to 0.175 mg/kg, in fruits ranged from 0.021 to 0.145 mg/kg and in vegetables ranged from 0.032 to 0.411 mg/kg, respectively. Hence, arsenic accumulation in cereals, pulses, oilseed, vegetables, spices, cole crop and fruits crop might not be safe in future without any sustainable mitigation strategies to avert the potential arsenic toxicity on the human health in the contaminated areas.
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