The dynamics of microvesicle formation in arterial blood in generalized Schwartzman phenomenon was studied. Successive (with 24-h interval) intravenous injections of endotoxin to rabbits in a dose of 1 mg/kg and 3 mg/kg caused an increase in the content of microvesicles in the blood, some of them containing ecto-5'-nucleotidase. Biphasic changes in arterial blood clotting time and erythrocyte hemolysis were observed.
Intravenous injection of 1 microg/kg desmopressin to rabbits not only accelerated clotting of arterial blood (maximally by 59.16 +/- 8.53% after 1 h), but also increased the number of microvesicles containing the integral enzyme 5'-nucleotidase in the arterial blood from the initial level of 36.26 +/- 8.08 ncat/liter to a maximum of 99.65 +/- 15.8 ncat/liter after 15 min.
The role of increased blood clotting in intravascular thrombus formation and, therefore, in the pathogenesis of many related diseases is currently beyond doubt. This is confirmed by the success of anticoagulant therapy for thromboembolic disease. A definite obstacle to the further spread of this method for prophylaxis in prethrombotic conditions is the difficulty in developing clear indications for its use. Many doctors in such cases rely on the analysis of the clinical features of the pathological process, as well as on the results of determining the blood coagulation rate, the Quick prothrombin index and some other indicators of the blood coagulation system [E. Perlik (1957), B. P. Kushelevsky (1958), N. N. Anosov and B. S. Vilensky (1959)].
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.