We have characterized a genomic clone of the mouse equivalent of alpha1-antitrypsin (alpha1-antiprotease) a gene that is expressed in liver. There are at least three other genomic sequences similar to the gene expressed in mouse liver and all appear to be located on chromosome 12. The duplicated regions might include genes that are also expressed in the liver and/or in other tissues. Comparison of the sequences of the mouse alpha1-antitrypsin sequence expressed in liver with sequences of alpha1-antitrypsins from other mammals reveals great homology in the coding regions, particularly in the amino acids at the active site, including the neighboring methionine and serine thought to play a key role in the protease inhibition.
Cholecystokinin (CCK) is a neuropeptide found in brain and intestine. In this report, we have isolated a cDNA clone that encodes CCK from a mouse brain cDNA library. This cDNA clone has extensive homology to CCK precursors that have been sequenced previously. Southern blots of genomic DNA probed with this cDNA clone revealed single bands for each of eight different restriction enzymes, all of which could be accounted for by a single genomic clone, suggesting that the CCK gene is present as a single-copy gene in mice. RNA blots, primer extensions, and S1 nuclease protection assays have suggested that the same RNA start site is utilized in brain and in gut. Finally, we have shown, by using RNA blots and a radioimmunoassay specific for CCK, that CCK is expressed at maximum adult levels in intestine at birth but that adult concentrations of CCK and its mRNA are not reached in brain until much later in development.
The stability of exogenous DNA microinjected into the cytoplasm at different stages of the meiotic cycle and after pronuclear formation was examined in ungulate species. Metabolism of the injected 1201 base pair (bp) DNA was examined by Southern blotting. Similar levels of metabolism of the injected DNA were detected in pig, sheep and bovine oocytes before germinal vesicle breakdown, in which about 30-40% of detected DNA was ligated into higher-molecular-weight forms. Porcine metaphase oocytes and pronuclear zygotes showed a reduced ability to ligate the exogenous DNA. In contrast, sheep and bovine metaphase oocytes and zygotes showed increased levels of ligation and, at the pronuclear stage, generated significant amounts of extremely large (greater than 15 kbp) ligation products. These results are discussed in the context of maternal precursors and metabolic activities in the egg.
In this paper we describe experiments that address specific issues concerning the regulation of the mouse cholecystokinin gene in brain and intestine. The mouse cholecystokinin gene was cloned and sequenced. Extensive homology among the mouse, man and rat genes was noted particularly in the three exons and the regions upstream of the RNA start site. RNAse protection assays for each of the three exons were used to demonstrate that CCK is expressed in only a subset of tissues and that the same cap site and splice choices are used in brain, intestine as well as in cerebellum, cortex, midbrain, hypothalamus and hippocampus. CCK RNA was also noted to be detectable in kidney. Thus the same gene using the same promoter is expressed in subsets of cells that differ in their biochemical, morphologic and functional characteristics. The level of expression of CCK was also monitored during mouse cortical development and the appearance of CCK RNA was compared to glutamate decarboxylase (GAD), enkephalin and somatostatin. It was noted that each of these cortical markers was first expressed at different times during cortical development. The appearance of CCK RNA during intestinal development was also measured and found to precede appearance in cortex by several days.
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