The objective of this study was to examine the effects of supplemental rumen-protected vitamin C (VC) on live and carcass-based performance, and antioxidant capacity of cattle consuming varying concentrations of dietary S. Angus-cross steers (n = 120) were blocked by initial BW (341 ± 11 kg) and assigned equally to 1 of 6 treatments, evaluating 3 concentrations of dietary S [0.22%, 0.34%, and 0.55%, for low S (LS), medium S (MS), and high S (HS), respectively] and 2 concentrations of supplemental VC (0 or 10 g • steer(-1) • d(-1)). Steers receiving VC-supplemented diets consumed an average of 10.3 g of supplemental VC • steer(-1) • d(-1) and increasing dietary S linearly increased (P < 0.01) grams of S consumed. Increasing dietary S decreased (P < 0.01) DMI, final BW, and ADG, and linearly increased (P < 0.05) rumen hydrogen sulfide and blood sulfhemoglobin concentrations. The inclusion of VC, regardless of S treatment, tended to increase (P = 0.08) plasma VC concentrations, specifically within the medium and high S diets (P = 0.04). Plasma total antioxidant capacity (d 90) linearly decreased (P = 0.003) and total liver glutathione (GSH; d 143) tended to decrease (P = 0.08) due to increased S intake. Within the high S treatment, addition of VC decreased (P = 0.04) the ratio of oxidized-to-reduced GSH compared with HS alone. Increased dietary S and VC decreased (P < 0.05) plasma Cu concentrations, whereas VC increased (P = 0.01) plasma Fe concentrations. Linear decreases (P < 0.02) in marbling score, backfat thickness (BF), yield grade, and HCW were observed as dietary S increased; however, the addition of VC to the HS diet increased (P < 0.01) BF, marbling scores, and percentage of cattle grading Choice compared with HS without VC. In conclusion, supplementation of VC to cattle receiving the high S diet improved marbling scores; although the exact mechanism for this improvement is unknown, it may be related to greater circulating VC available for lipid metabolism in these cattle.

ABSTRACT:While many cattle feeding areas in the United States have long dealt with high sulfate water, increased feeding of ethanol co-products such as distillers grains with solubles to beef cattle has led to a corresponding increase in dietary sulfur. As a result, sulfur metabolism in the ruminant has been the focus of many research studies over the past ten years, and advances in our knowledge have been made. Excessive sulfur in cattle diets may have implications on trace mineral absorption, dry matter intake, and overall cattle growth. This review will focus on what we have learned about the metabolism of sulfur in the ruminant, including ruminal sulfate reducing bacteria, the role of ruminally available sulfur, factors affecting the production of hydrogen sulfide in the rumen, and the potential mechanisms behind sulfur toxicity in cattle.Additionally, this review will discuss potential strategies to minimize risk of sulfur toxicity when cattle are fed high-sulfur diets, including dietary and management strategies. Further research related to high-sulfur diets including implications for carcass characteristics, meat quality, and animal health will also be discussed. As ethanol production processes continue to change, the nutrient profile of the resulting co-products will as well. Often removal of one nutrient such as oil will result in the concentration of other nutrients such as sulfur. Thus it seems even more likely that a better understanding of sulfur metabolism in the ruminant will be important to beef cattle feeding in the future.

To examine the effects of dietary S on diet digestibility and apparent mineral absorption and retention, 16 steers [8 ruminally fistulated (368 ± 12 kg BW) and 8 unmodified (388 ± 10 kg BW)] were paired within modification status and BW, and within each of the 2 consecutive 28-d periods, 4 pairs of steers were randomly assigned to either a low-S (0.24%) or high-S (0.68%) pelleted diet. Bromegrass hay was fed at 5 or 7% of the diet, during periods 1 and 2, respectively. Sodium sulfate was used to increase the S content of the high-S diet. The low-S steers were fed the amount of feed their high-S counterpart consumed the previous day, while the high-S steers received 110% of the previous day's intake. Steers were adapted to individual metabolism stalls for 4 d (d -3 to 0 of period), acclimated to diet for 7 d (d 1 to 7 of period), and after high-S steers were consuming ad libitum intake for 7 d (d 14 of period), total urine and feces were collected for 5 d. Feed intake and orts were recorded daily. Dry matter and OM digestibility were determined. Jugular blood was collected before and after each collection period on d 14 and 20, and liver biopsies were collected on d 0 and 27. Macromineral (Ca, K, Mg, and Na) and micromineral (Cu, Mn, and Zn) concentrations were determined for pellets and hay, orts, feces, urine, and plasma and liver samples from each steer via inductively coupled plasma spectrometry. Dry matter intake, DM and OM digestibility, and urine volume were not affected (P ≥ 0.11) by dietary treatment, but fecal output was greater (P = 0.02) in the low-S steers than the high-S steers. A high-S diet decreased plasma Cu (P = 0.04) and liver Zn (P = 0.03) compared to low-S steers. No differences (P ≥ 0.20) were noted among urinary excretion of Cu, Mn, and Zn. Sodium absorption was greater (P < 0.01) and Cu, Mn, and Zn retention was lesser (P ≤ 0.01) in the high-S steers than the low-S steers. Apparent absorption of Ca, K, and Mg was not affected (P ≥ 0.18) by dietary treatment, while absorption of Cu, Mn, and Zn in the high-S treatment was lesser (P ≤ 0.06). In conclusion, consumption of a high-S diet for 28 d had limited effects on Ca, K, Mg, and Na absorption and retention, but decreased Cu, Mn, and Zn retention, which may limit growth and production of cattle consuming a high-S diet long-term.

To examine the effects of cattle breed on the clearance rate of an injectable mineral product, 10 Angus and 10 Simmental steers were blocked by breed and initial BW (332 ± 33 kg) and injected with either Multimin 90 (MM) or sterilized saline (CON) at a dose of 1 mL/45 kg BW. Multimin 90 contains 15 mg Cu/mL (as Cu disodium EDTA), 60 mg Zn/mL (as Zn disodium EDTA), 10 mg Mn/mL (as Mn disodium EDTA), and 5 mg Se/mL (as sodium selenite). Steers received a corn-silage-based diet, and inorganic sources of Cu, Zn, Mn, and Se were supplemented at NRC recommended amounts. Jugular blood was collected immediately before injection and at 8 and 10 h post-injection and on days 1, 8, and 15 post-injection. Liver biopsies were collected 3 d before injection and on days 1, 8, and 15 post-injection. Liver and plasma mineral concentration and glutathione peroxidase (GSH-Px) activity data were analyzed as repeated measures. Plasma concentrations of Zn, Mn, and Se were greater (P = 0.01) and Cu tended to be greater (P = 0.12) post-injection in MM steers compared with the CON steers. Regardless of treatment, Simmental cattle had lower plasma concentrations of Cu, Zn, and Se (P ≤ 0.05) when compared with Angus cattle. Erythrocyte GSH-Px activity was greater (P = 0.01) in MM steers compared with CON steers. Liver concentrations of Cu, Zn, and Se were greater (P = 0.05) in MM steers compared with CON steers post-injection. Liver Mn concentrations tended to be greater (P = 0.06) in MM steers compared with CON steers in the days post-injection. Interestingly, Simmental cattle exhibited greater (P = 0.01) liver Mn concentrations in the days after injection compared with Angus cattle (7.0 and 6.0 mg Mn/kg for Simmental and Angus cattle, respectively), regardless of treatment. It is unclear if this breed difference is biologically relevant; however, these data may suggest that differences in liver excretion of Mn exist between the two breeds. Overall, use of an injectable trace mineral increased liver concentrations of Cu and Se through the 15-d sampling period, suggesting that this injectable mineral is an adequate way to improve Cu and Se status of cattle through at least 15 d.

The objective of this study was to examine the effects of vitamin C (VC) supplementation for an average of 102 d before harvest on finishing performance and blood metabolites of steers receiving a 40% dry distillers grains plus solubles diet (0.55% S). Yearling, Angus-cross steers (n = 140) were blocked by initial BW (432 ± 25.5 kg), stratified within blocks by intramuscular fat (3.6% ± 0.30%) determined by ultrasonography, and assigned to treatments (5 steers/pen, 7 pens/treatment). Treatments included 1) no VC control (CON), 2) 5 g VC • steer(-1) • d(-1) (5VC), 3) 10 g VC • steer(-1) • d(-1) (10VC), and 4) 20 g VC • steer(-1) • d(-1) (20VC). Jugular blood was collected from 2 steers/pen before feeding at the beginning and end of the 102-d study, and steers were harvested by block on 3 separate dates (d 91, 105, and 112). Sulfur intake linearly decreased (P = 0.01) as VC inclusion increased (59.2, 57.7, 57.0, and 54.8 ± 0.79 g S • steer(-1) • d(-1) for CON, 5 VC, 10 VC, and 20 VC, respectively). The CON cattle had greater (P < 0.01) DMI than the VC-supplemented cattle. Inclusion of VC did not influence ADG or final BW, resulting in a tendency for a linear increase (P = 0.08) in G:F as VC inclusion increased (0.150, 0.152, 0.158, and 0.160 ± 0.004 for CON, 5 VC, 10 VC, and 20 VC, respectively). Ending (2 d before harvest) plasma ascorbate showed a quadratic effect (P < 0.05) because of lesser concentrations exhibited by 5 VC cattle (1,186 µg/L) compared with the CON (1,454 µg/L), 10 VC (1,304 µg/L), and 20 VC (1,436 µg/L; SEM ± 64.8) cattle. Ending plasma insulin concentrations of CON cattle tended (P = 0.07) to be less than the VC-supplemented cattle. Plasma glucose and NEFA concentrations were not affected (P ≥ 0.23) by VC inclusion. Hot carcass weight, 12th-rib back fat, marbling, and quality grade were not affected (P ≥ 0.27) by VC inclusion. Increasing VC inclusion linearly increased (P = 0.02) rib eye area (84.9, 86.5, 88.7, and 89.1 cm(2) ± 1.17 for CON, 5 VC, 10 VC, and 20 VC, respectively), corresponding to a linear decrease (P = 0.02) in yield grade with increasing inclusions of VC. A tendency (P = 0.06) for a quadratic effect on KPH was observed, in which values generally increased from CON (2.27%) to 5 VC (2.37%) to and 10 VC (2.39%), then decreased in 20 VC (2.20%). In conclusion, VC supplementation to a high-S diet for an average of 102 d before harvest has limited effects on blood metabolites but increased rib eye area and tended to increase feed efficiency of yearling steers.

The objective of this study was to determine the effects of adding ferric ammonium citrate (FAC; 300 mg ferric Fe/kg DM) to a 0.51% S diet on diet digestibility, mineral balance, and 56-d performance of steers fed a high concentrate diet. Angus-crossbred steers (n = 18) were randomly assigned to 1 of 3 treatments: 1) control diet (0.21% S; CON), 2) CON + sodium sulfate (0.51% S; high S [HS]), and 3) the HS diet + 300 mg of ferric Fe from FAC/kg DM (0.51% S; HS+Fe). This study included 2 phases, 1) a metabolism trial (d -1 to 20) and 2) a 56-d feedlot trial (d 22 to 79). In phase 1, 2 groups of 9 steers (370 ± 9.5 kg) were adapted to diet (10 d) and metabolism stalls (5 d), and following the adaptation period, a 5-d total collection of feces and urine was conducted. Feed offered and refused was recorded daily, and diet digestibility and retention of Cu, Fe, Mn, and Zn was determined. In phase 2, steers (384 ± 11.9 kg) were individually fed their respective diet in feedlot pens for 56 d and ADG was determined. From each steer, jugular blood and a liver biopsy were collected on d -1, 41, and 72 and d -1 and 72, respectively, for mineral content. Ruminal hydrogen sulfide concentrations (n = 18) were determined on d -1, 9, 23, 31, 41, 51, 61, and 72. In phase 1, DMI (P = 0.02), fecal output (P = 0.06), and intake of Cu, Mn, and Zn (P ≤ 0.04) were less in steers consuming the high S diets than CON, but DM and OM digestibility and urine excretion of minerals were not different (P ≥ 0.12) due to treatment. As a percent of intake, urinary excretion of Cu tended (P = 0.07) to be greater in the HS steers than the CON and HS+Fe steers, which did not differ (P = 0.74). In phase 2, BW, ADG, and G:F were not different (P ≥ 0.29) due to treatment, but DMI was lesser (P < 0.01) in the HS+Fe steers than CON and HS steers, which did not differ (P = 0.13). Ruminal hydrogen sulfide concentrations were greater (P < 0.01) in the steers fed high S diets than CON but were not different (P = 0.86) among the high S diets. Plasma Cu, Fe, and Zn concentrations were not different (P ≥ 0.27) due to treatment on all days. Final liver Cu concentrations were lesser (P < 0.01) in the steers fed high S diets compared with the CON, while liver Fe, Mn, and Zn concentrations did not differ (P ≥ 0.28) among treatments. In conclusion, adding Fe to a high S diet did not affect DM or OM digestibility or trace mineral absorption and status of steers relative to the HS diet alone.

No abstract

The data support the conclusion that vitamin C (VC) supplemented to feedlot cattle consuming high sulfur (S) diets may increase tenderness of the beef longissimus dorsi by protecting the protease µ-calpain. Additionally, VC supplementation improved the fatty acid profile of meat products by increasing omega 3 and omega 6 fatty acids (FA) and decreasing saturated fatty acids (SFA).