The effect of incubation temperature (2, 4, 6, 8 and 10 C) on haddock Melanogrammus aeglefinus development and growth during the embryonic period and in subsequent ontogeny in a common post-hatch thermal environment (6 C) was investigated. Hatching times were inversely proportional to incubation temperature and ranged from 20Á3 days at 2 C to 9Á1 days at 10 C. Growth rates were directly proportional to incubation temperature during both the embryonic and larval periods. There was a significant decline in growth rates following hatch in all temperature groups. Compared to the endogenously feeding embryos, growth rates in the exogenous period declined by 4Á4-fold at 4 C to 3Á9-fold at 8 C, indicative of the demarcation between the endogenous and exogenous feeding periods. Yolk utilization varied from 17 days at 2 C to 6 days at 10 C and followed a three-stage sigmoidal pattern with the initial lag period inversely proportional to incubation temperature. Time to 50% yolk depletion varied inversely with temperature but occurred 1-1Á5 days post-hatch at all temperatures. Additionally, the period between 10 and 90% yolk depletion also decreased with increased temperature. Overall developmental rate was sequential with and directly proportional (2Á3-fold increase) to incubation temperature while the time spent in each developmental stage was inversely proportional to temperature. Larger embryos tended to be produced at lower temperatures but this pattern reversed following hatch, as larvae from higher temperature groups grew more rapidly than those from other temperature groups. Larvae from all temperatures achieved a similar length (c.total length 4Á5 mm) upon complete yolk absorption. The study demonstrated the significant impact that temperature has upon developmental and growth rates in both endogenous and exogenous feeding periods. It also illustrated that temperature changes during embryogenesis had significant and persistent effects on growth in subsequent ontogeny. # 2005 Fisheries and Oceans canada

Muscle development and growth were investigated in haddock larvae(Melanogrammus aeglefinus L.) incubated under controlled temperatures(4, 6, 8°C) and reared post-hatch through yolk-dependent and exogenous-feeding stages in a 6°C post-hatch environment. Changes in cell number and size in superficial and deep myotomes within the epaxial muscle were investigated for 28 days following hatch. Distinct and significant differences in muscle cellularity following separate developmental strategies were observed in superficial and deep myotomes. The number of superficial myofibres increased with time and, although not in a manner proportional to temperature during the first 21 days post hatch (d.p.h.), there was observed a trend during the final 7 days of greater mean cell size that was strongly associated with increased temperature. In addition, there was an apparent correspondence between increased temperature and increased size between 21 and 28 d.p.h. Among all temperature groups the superficial myotome not only demonstrated a consistent unimodal myofibre-size distribution but one that increased in range proportional to temperature. In the deep muscle, myotomes from higher incubation temperatures had a broader range of fibre sizes and greater numbers of myofibres. The onset of a proliferative event,characterized by a significant recruitment of new smaller myofibres and a bimodal distribution of cell sizes, was directly proportional to incubation temperature such that it occurred at 14 d.p.h. at 8°C but not until 28 d.p.h. at 4°C. The magnitude of that recruitment was also directly proportional to temperature. Following hatch, those embryos from the greatest temperature groups had the largest mean deep muscle size but, as a result of the proliferative event, had the smallest-sized cells 28 days later. The muscle developmental and growth strategy as indicated by sequential changes in cellularity and cell-size distributions between myotomes in response to temperature are also discussed in light of whole animal growth and development.

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Prey exploitation was documented for Hippoglossoides platessoides, Pleuronectes ferrugineus, and P. americanus collected from southeast Sable Island Bank in February and June 1989. Diets varied significantly by sample and with fish species and length. Of 239 species consumed by flatfishes, 66 were determined to be principal prey. Crustaceans, particularly amphipods, were the most frequently exploited prey of all three flatfish species. Small H. platessoides fed on suprabenthic fauna, while larger fish exploited epifauna and hyperiid suprafauna. P. ferrugineus exploited epibenthic fauna, consuming crustaceans and tunicates when small and polychaetes when larger. P. americanus preyed on epifauna; smaller fish exploited polychaetes while larger fish usually consumed crustaceans associated with ectoproct colonies. Feeding intensity was dramatically lower in February, food being found in stomachs of only 36% of H. platessoides and 49% of P. ferrugineus, and lacking in the stomachs of P. americanus. In June, stomachs of 86% of H. platessoides, 94% of P. ferrugineus and 96% of P. americanus contained food. Results indicate that prey resources for the three flatfish species are partitioned by specific habitat and prey type preferences.

Cell lines can be useful experimental tools for studying marine fish, which are often difficult to routinely obtain and maintain in the laboratory. As few cell lines are available from coldwater marine fish, cultures were initiated from late gastrula embryos of haddock (Melanogrammus aeglefinus) in Leibovitz's L-15 with fetal bovine serum (FBS). From one culture, a cell line (HEW) emerged that has been grown for close to 100 population doublings, was heteroploid, and expressed telomerase activity, all of which suggest HEW is immortal. Growth occurred only if FBS was present and was optimal at 12 to 18 degrees C. Usually most cells had an epithelial-like morphology, but under some conditions, cells drew up into round central bodies from which radiated cytoplasmic extensions with multiple branches. These neural-like cells appeared within a few hours of cultures being placed at 28 degrees C or being switch to a simple salt solution (SSS). At 28 degrees C, cells died within 24 h. In SSS, HEW cells survived as a monolayer for at least 7 days. The sensitivity of HEW cells to morphological change and their capacity to withstand starvation should make them useful for investigating cellular responses to environmental stresses.

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