SUMMARY :The experiment was carried to screened cotton genotypes for salinity tolerance in the kharif season of 2016-17. Eleven cotton genotypes were selected and grown upto squaring stage in different soil ratio of normal soil and saline soil after that leaf samples were collected and all the biochemical parameters were recorded for screening of cotton genotypes for salinity tolerance. Genotypes GISV-218 and G. Cot-16 showed highest relative water content in all the treatment. Genotypes G.Cot-16, GISV-218 and BC-68-2 showed highest membrane stability in normal soil condition where genotypes G. Cot -16, GISV-218 and 76-1H-20 were showed highest membrane stability (78.79%, 78.29% and 74.70%, respectively) in saline soil. Range of proline content was (0.23 to 0.56 mg/g of tissue) in normal soil condition and it was (0.75 to 1.35 mg/g of tissue) in saline soil condition. Highest glycine
Molecular markers, particularly, STS markers are highly specific for regions of DNA being used for marker assisted selection (MAS). The present study was carried out with the objective to validate the STS markers, previously reported to be linked with fertility restorer (Rf1) gene for CMS lines of cotton (Gossypium hirsutum). Four sets of primer pairs viz., UBC 147 1400 , UBC607 500 , UBC679 700 and UBC659 1500 were used to identify restorer and non-restorer lines of G. hirsutum with G. harkenssii background. Out of the four marker sets screened, UBC607 500 showed confirmatory results by amplifying a single band of size 517 bp in restorer lines and no amplification product was obtained in any of the non-restorer plants by these markers. Other STS markers, however, failed to show confirmatory results.Keywords G. hirsutum Á MAS Á Restorer genes Á STS markers Cytoplasmic male sterility (CMS) is a maternally inherited trait conferring the inability to produce functional pollen because of interaction between cytoplasmic and nuclear genes. Since CMS does not affect female fertility, male sterile plants are able to set seeds as long as viable pollens are provided. The presence of certain nuclear genes, Rf (Restoring fertility), can effectively suppress the malesterile cytoplasm and restore pollen fertility. The application of CMS/Rf system has proved to be an effective means to produce commercial F1 hybrid seed for many crops (Williams 1992). Cotton is predominantly a self-pollinated crop, however, cotton breeders have been trying to breed F1 hybrid cotton, to harness F1 heterosis for many desirable traits such as high seedling-vigor, earliness, superior fiber quality and yield (Davis 1978). Before the introduction of the CMS/Rf system into Upland cotton, the only way to generate commercial hybrid cotton was through hand emasculation and crossing, but due to high labor costs, hand-crossing made the price of hybrid cotton prohibitive. The first CMS line of commercial cotton was introduced by crossing an upland cotton, G. hirsutum, as male parent, to a wild species, G. harknessii (Meyer 1973). A Rf line was also developed by transferring a nuclear restorer gene from G. harknessii into G.hirsutum simultaneously (Meyer 1975). The F1 hybrid population generated by crossing the CMS line to the Rf line showed a wide range of male fertility expression. Several CMS/Rf lines were then developed through the backcross method (Weaver and Weaver 1977). Identification of molecular markers closely linked to the nuclear Rf genes could help breeders to distinguish restorer and non-restorer plants at early seedling stage without crossing them to CMS lines. The present investigation was, therefore, conducted to validate the STS markers closely linked to the nuclear Rf gene under male sterile cytoplasm in commercial cotton.The experimental material comprised of four restorer lines of G. hirsutum viz., 8-1029 (G. harkenssii based), SRT restorer-3, SRT restorer-12 and SRT restorer-13 (developed by back crossing with 8-1029 restorer lines) as ...
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