A pseudoepithelial pattern was observed in smears prepared from fine needle aspirates (FNA) from three plasmacytomas. In one case cells displayed a markedly granular, 'oncocytic' cytoplasm. Granularity of the cytoplasm was due to a large number of mitochondria as shown by electron microscopy. B-lymphocytic origin of the neoplasms was confirmed by immunocytochemistry.
Electron-microscopic examination of peripheral blood from a patient with ‘hairy cell’ leukemia revealed classical ‘hairy cells’, atypical lymphoid cells and numerous pathological plasma cells. Osmiophilic granular material coated the cell surface of ‘hairy cells’ and lymphoid cells but not the plasmalemma of the plasma cells. The most important features of the plasma cells were cytoplasmic protrusions and masses of oncogenic virus A particles in their endoplasmic reticular cisternae.
The interaction of chrysotile and amphibole asbestos fibers with the cytoskeleton of cultured human mesothelial cells from nontumoral pleural effusions was studied using scanning electron and immunofluorescence microscopy. Asbestos-exposed mesothelial cells show a massive annular condensation of cytokeratin filaments, forming a concentric ring enveloping the nucleus and the phagocytosed asbestos fibers. By detergent extraction of the cells it could be shown that the asbestos fibers are in close contact with the nuclear membrane and associated with the cytoskeletal framework of the cells. An association of cytokeratin filaments with the asbestos could be observed during phagocytosis of the fibers. The disturbance of the cell cytoskeleton and the close morphologic contact between asbestos fibers and the nuclear membrane may have some relevance in explaining the well-recognized carcinogenic effects of asbestos mineral fibers.
Cells from the peripheral blood of a patient with ‘hairy cell’ leukemia were cultivated in long-term cultures. They grew with a monolayer growth pattern and consisted of at least two cell populations. The electron microscopic morphology of the cultivated cells revealed cells resembling ‘hairy cells’ showing the typical cytoplasmic protrusions and cells with structural characteristics of plasma cells.
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