Occurrence of Plasmocytoid Cells in Long-Term Blood-Cell Cultures from a Patient with ‘Hairy Cell’ Leukemia

Abstract

Cells from the peripheral blood of a patient with ‘hairy cell’ leukemia were cultivated in long-term cultures. They grew with a monolayer growth pattern and consisted of at least two cell populations. The electron microscopic morphology of the cultivated cells revealed cells resembling ‘hairy cells’ showing the typical cytoplasmic protrusions and cells with structural characteristics of plasma cells.

Silica Toxicity for Macrophages <i>in vitro: </i>a New Cytotoxicity Test

“…Attachment to feeder cells and peripolesis was checked with an allogeneic fibroblas tic monolayer cell culture. For electron microscopy, cells were centrifuged at 400 g, the pellet resuspended and processed as previously described [16]. The main cell characteristics were typical for long-term cultures of haematopoietic origin, namely a selection of lymphocytoid-cell-type with pleomorphic morphology [21].…”