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Nerve growth factor (NGF) treatment converts rapidly dividing PC12 cells into a neuronal phenotype. To understand the Ca2+ sequestration mechanisms accompanying this differentiation, we examined the endoplasmic reticulum Ca2+ (SERCA) pump levels using two different assays: ATP-dependent azide insensitive oxalate stimulated 45Ca2+ uptake by PC12 cells permeabilized with saponin, and Western blots using a monoclonal antibody which reacts with all the SERCA isoforms. We also examined the reaction to an antibody against the plasma membrane Ca2+ (PMCA) pump. NGF treatment decreased the SERCA pump expression but it increased the PMCA pump level. These results are consistent with a greater role of PMCA pumps in neuronal cells than in most other cells and with an increased role of SERCA pumps during cell proliferation.

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were highly significant (P < 0.01), only measurements taken within 1 mo of completing the growth-performance test were sufficiently predictable for culling 1-yr-old bulls. Correlation coefficients calculated between SC in 1-yr-old bulls and SC and PTW when they were 2 yr old were 0.76 and 0.65 (P < 0.01).These values suggest that SC in young beef bulls is a useful predictor of PTW.Also, this study showed that testis size of l-yr-old bulls differed among breeds and that SC of l-yr-old bulls can be used as an aid to select herd sires likely to have above-average testicular size, thereby increasing the probability of impregnating more females when under heavy breeding pressure.On a 6tudi6 le rapport entre le poids des testicules (PTW)

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