The aim of this study was to evaluate the serologic status of domestic pigeons not infected and asymptomatically infected with the pigeon circovirus (PiCV) with the use of an enzyme-linked assay based on PiCV recombinant capsid protein as a plate antigen. Recombinant PiCV capsid protein was produced by transforming E. coli BL21 (DE3) Rosetta colonies with expression plasmids.Blood samples and cloacal swabs were collected from 171 asymptomatic pigeons. The birds were divided into two groups (infected and not infected with PiCV) based on the results of Sybr Green real time PCR screening for the presence of PiCV genetic material. Approximately 70% of the pigeons tested positive for anti-PiCV antibodies regardless of their infection status. Antibody levels, the coefficient of variation and standard deviation were significantly higher in the group of infected pigeons.The results indicate that ELISA is a highly useful test that complements molecular methods in evaluations of PiCV infection status in domestic pigeons. The spread of pigeon circovirus infections can be controlled by keeping breeding flocks free of PiCV, which can only be achieved by subjecting birds to real time PCR and serological tests.

Chlamydiosis is a zoonotic disease caused by Chlamydia psittaci that occurs in a wide range of bird species. High infection rates with C. psittaci are found in pigeons, which can act as vectors transmitting this bacterium to poultry and humans. Chlamydia shedding by pigeons is intermittent and can be activated by stressors or immunosuppression. The most common immunosuppressive factor for pigeons is a pigeon circovirus (PiCV) infection. The main aim of the study was to evaluate the prevalence of C. psittaci in Polish populations of domestic and feral pigeons (Columba livia) in the context of its correlation with PiCV infections. The second objective was to determine the genetic characteristics of Polish C. psittaci isolates. The study was conducted on 377 pigeon samples (276 domestic and 101 feral pigeons) collected from pigeons from different regions of Poland. The average prevalence of C. psittaci in the Polish pigeon population was determined at 6.8%, and it was higher in domestic than in feral pigeons. This is the first ever study to suggest a potential correlation between C. psittaci and PiCV infections, which could be attributed to the fact that there are 2 to 3 times more pigeons infected with C. psittaci and coinfected with PiCV than pigeons infected with C. psittaci alone. This trend was observed mainly in the population of sick pigeons. As many as 88.2% of isolates were recognized as belonging to genotype B, and the remaining isolates were identified as belonging to genotype E. The isolates analyzed in this study demonstrated low levels of genetic variation (96-100% homology among the isolates and in relation to reference strains). Chlamydia psittaci could be expected to spread across pigeon populations due to the high probability of mutual infections between birds and the increasing number of PiCV infections.

The aim of this study was to develop international standards for evaluating strength endurance with the use of the 3‐Minute Burpee Test. The results of 3862 women (Poland – 2502, Great Britain – 500, Hungary – 412, Serbia – 448) and 5971 men (Poland – 4517, Great Britain – 500, Hungary – 451, Serbia – 503) aged 18‐25 (mean age of 20.36 ± 0.94 and 20.05 ± 1.25 y, respectively) were collated between 2004 and 2018. The students’ strength endurance was evaluated in the 3‐Minute Burpee Test. The results were expressed on a uniform scale with the 3‐sigma rule which was used to develop the T‐score scale for the 3‐Minute Burpee Test. Men completed 56.69 cycles/3 min and women – 48.84/3 min on average. The best male participant completed 82 burpees, and the best female participant – 73 burpees. The majority of male and female participants (66.71% and 68.18%, respectively) were characterized by average strength endurance in the 3‐Minute Burpee Test (range of scores: 47‐66 and 37‐60 cycles/3 min, respectively). Very good strength endurance (76‐85 and 72‐83 cycles/3 min, respectively) was noted in the smallest percentage of male and female participants (0.52% and 0.26%, respectively). Similar studies should be carried out in other countries and in different age groups to develop objective international classification standards for variously‐aged individuals.

Aim: This article presents cross-sectional data collected from the population of first-year students at the University of Warmia and Mazury in Olsztyn in 2000-2016. The aim of the study was to investigate changes in the following trends among university students after Poland's accession to the European Union (2004): (1) alcohol consumption, (2) alcoholic beverage preferences, and (3) attitudes towards alcohol. Method: The study relied on data from anonymous surveys conducted every two years between 2000 and 2016 among a representative sample of first-year university students. A total of 9778 individuals (4264 males and 5514 females) completed anonymous questionnaires during the period under analysis. Statistical analyses were performed by comparing the frequency of answers given by male and female students in the first and last year of the study. Results: There was a steady linear decrease in the percentages of abstainers of both genders and a regular linear increase in the number of female students abusing alcohol to blackout level in the analysed period of 2000 to 2016. The respondents' preferences for alcohol brands became more

IntroductionMethotrexate (MTX) has anti-proliferative and anti-inflammatory effects in psoriasis. Moreover, low doses can reduce the risk of developing cardiovascular diseases. It turns out that psoriasis and atherosclerosis have a similar pathogenetic mechanism: the same pro-inflammatory cytokines, Th1 and Th17, are involved in both diseases.AimTo evaluate the effects of metabolic markers, protective cytokines (interleukin 10 (IL-10), transforming growth factor β (TGF-β)) and a marker of endothelial damage (endocan) in patients with plaque psoriasis.Material and methodsThe study included 24 patients aged 27–69 years (9 female, 15 male) with plaque psoriasis. The metabolic syndrome according to the International Diabetes Federation (IDF) was evaluated. The laboratory tests were performed under fasting conditions: C-reactive protein (CRP), glucose, total cholesterol, triglycerides, high-density lipoprotein (HDL), uric acid, endocan, IL-10, and TGF-β. After 12 weeks of treatment with MTX injections 15 mg/week, every patient was assessed with the same laboratory tests.ResultsAfter treatment we observed a statistically significant increase of endocan and IL-10, but no significant differences in the titer of TGF-β. C-reactive protein was reduced by approximately 54.7%. No improvement of lipid profile was observed, and even a significant increase in triglycerides was noted. Similarly, no significant difference was seen in the case of glucose and uric acid prior to and after treatment.ConclusionsMethotrexate in low doses in short-term treatment decreases CRP (anti-inflammatory effect) and increases endocan and IL-10 (potential protective role). Methotrexate is characterized by good efficacy and tolerability in therapy of patients with psoriasis.

Relationships between the physical properties of seed and their germination capacity and energy is an important consideration for separating seeds of the highest or lowest quality. The objective of this study was to determine the correlations between the selected aerodynamic, geometric, frictional and mass parameters of cultivated radish (Raphanus sativus L.) seeds and their germination capacity for the purpose of improving seed quality. The parameters were determined for graded and discarded radish seeds and impurities were separated by a gravity separator. A germination test was performed, its results were verified every 12 hours, and the germination rate index was determined. A statistical analysis revealed that germinated and non-germinated seeds from the graded fraction did not differ significantly. In the discarded fraction, germinated seeds were characterized by higher terminal velocity (by approx. 10%), thickness (by approx. 9%) and mass (by approx. 26%) in comparison with non-germinated seeds. Significant positive correlations were noted between the germination time vs terminal velocity and mass of discarded seeds. Thus, the separation of radish seeds should take place in two stages using a gravity separator and a pneumatic separator. A vertical stream of air with the speed of approx. 8 m s -1 allows recovery of around 37% of discarded seeds that contain approx. 86% of healthy germs. The resulting seed mixture is characterized by greater germination energy and capacity than the seed fraction graded on a gravity separator only.

The aim of the study was to evaluate the relationship between body mass index (BMI) and body mass loss (BML) induced by thermal stress in a dry sauna. The study was conducted on a group of 674 sedentary students, 326 women and 348 men aged 19-20. The correlations between BMI scores and BML were determined. The subjects were placed in supine position in a dry sauna for two sessions of 10 minutes each with a 5-minute break. The influence of BMI on the amount of BML in the sauna was determined by nonlinear stepwise regression. The smallest BML was noted in underweight subjects; students with normal weight lost more weight, whereas the greatest BML was reported in overweight and obese subjects. Persons with a high BMI are at higher risk of dehydration, and they should pay particular attention to replenishing fluids during a visit to the sauna. The proposed equations for calculating BML based on a person's BMI can be useful in estimating the amount of fluids that should be replenished by both men and women during a visit to a dry sauna.

The research findings call for a specially designed pro-health programme to be implemented during the time of the studies in order to raise the female students' awareness of leading a health-conscious lifestyle.