SummaryThe leukocyte integrin LFA-1 (CD1 la/CD 18) plays an important role in lymphocyte recirculation and homotypic interactions. Leukocytes from mice lacking CD11a displayed defects in in vitro homotypic aggregation, in proliferation in mixed lymphocyte reactions, and in response to mitogen. Mutant mice mounted normal cytotoxic T cell (CTL) responses against systemic LCMV and VSV infections and showed normal ex vivo CTL function. However, LFA-l-deficient mice did not reject immunogenic tumors grafted into footpads and did not demonstrate priming response against tumor-specific antigen. Thus CD11a deficiency causes a selective defect in induction of peripheral immune responses whereas responses to systemic infection are normal.
HLA-DQ plays an important role in the activation of SMX-NO-specific CD4+ T cells. Detection of HLA-DQ allele-restricted responses suggests that T cells are activated by a limited repertoire of SMX-NO-modified peptides.
A mouse anti-guinea pig monoclonal antibody, designated MSgp 2, was derived by the fusion of NS-1 myeloma cells with mouse splenocytes hyperimmunized with guinea pig B cells. MSgp 2 reacts with an antigen present on the majority of lymphocytes. An unexpected finding was the expression of the MSgp 2 antigen on epidermal Langerhans cells, as defined by cell morphology, expression of MHC class II antigen and presence of Birbeck granules in positive cells. It is suggested that the tissue distribution of MSgp 2 antigen on lymphocytes of the lymph node could indicate a role for this determinant in cell migration.
We investigated whether guinea pigs sensitized to ovalbumin show changes in T cells in the bronchial wall and whether they correlate with eosinophil migration. Animals received two injections of 10 µg ovalbumin in Al(OH)3 with a 2-week interval. Lungs were resected and frozen and cryostat sections stained with monoclonal antibodies recognizing T cells and subsets. Eosinophil peroxidase staining was used to identify this cell type. Sections stained with each marker were read ‘blind’ and cells enumerated in the bronchial lamina propria. A large number of T cells, mainly of CD4+ subset, were recruited into the bronchi 7 days after the booster injection of the antigen, in contrast to nonimmunized or nonboosted animals. These changes were also accompanied by an infiltration of eosinophils in boosted animals and suggest that T cells and/or their products play an important role in the development of bronchial hyperreactivity in this model.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.