An Antigenic Determinant Common to Lymphocytes and Langerhans Cells of the Guinea Pig

Healey, Dan; Baker, D.; Gschmeissner, Steve; Turk, J.L.

doi:10.1159/000234176

Cited by 9 publications

(7 citation statements)

References 14 publications

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“…In epidermal sheet preparations, the anti-la reagents revealed a si milar distribution to that reported in the human and mouse, where staining was found predominantly on the cell body [24], This was revealed by double label ling of the cells with CT4, which showed the Langer hans cell to be a highly dendritic cell. This confirmed our previous observation with the antibody MSgp2 which appears to detect a similar epitope to CT4 [13]. CT4 has been described to detect a homing receptor found on guinea pig lymphocytes which facilitates migration across high endothelial venules [16], High endothelial venules have been reported to occur in the skin [25] and Langerhans cells have been shown to be derived from a mobile pool of bone marrow-derived precursors [3].…”

Section: Discussionsupporting

confidence: 88%

“…As in other species, guinea pig Langerhans cells express MHC class-II antigens. At the ultrastructural level these MHC class-II-positive cells have the features of Langerhans cells, but many do not contain Birbeck granules [13]. In epidermal sheet preparations, the anti-la reagents revealed a si milar distribution to that reported in the human and mouse, where staining was found predominantly on the cell body [24], This was revealed by double label ling of the cells with CT4, which showed the Langer hans cell to be a highly dendritic cell.…”

Section: Discussionmentioning

confidence: 79%

“…A single epidermal cell suspension was prepared from guinea pig ear skin [13] and cytospin preparations were made and acetone fixed [14], 5-mm cryostat sections were prepared from gui nea pig ear skin [15].…”

Section: Preparation Of Tissuesmentioning

confidence: 99%

“…Sections and cytospin preparations and epidermal sheets were stained as described previously [13,15]. Briefly, the sheets were in cubated with the primary antibody (table I) overnight at 4°C.…”

Section: Staining Of Tissuesmentioning

confidence: 99%

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Phenotypic Analysis of Guinea Pig Langerhans Cells with Antibodies Directed against Leucocyte Surface Antigens

Baker

Healey

Verghese

et al. 1988

Int Arch Allergy Immunol

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The epidermis was stained with a panel of recently produced anti-guinea pig leucocyte antibodies. Guinea pig Langerhans cells were not detectable with antibodies directed against B lymphocytes (MSgp9), T lymphocytes (CT7 and MSgp7), T-helper/inducer (MSgp12) and putative T-suppressor/cytotoxic (CT6 and MSgp6) subsets. Langerhans cell expressed both major histocompatibility complex (MHC) class-I and II antigens and also an epitope (CT4) associated with lymphocyte migration, thus suggesting the migratory potential of this cell. Although the Langerhans cell did not express macrophage specific antigens, MSgp5, which detects lymphoid dendritic cells, was weakly expressed on the Langerhans cell. The Langerhans cell expressed a leucocyte-common antigen detected by H201. Double-labelling studies with anti-MHC class-II antibodies indicated that only 0.4 ± 0.3% of the pan leucocyte-positive epidermal cells were Ia-negative, indicating that it is unlikely that a guinea pig analogue of the murine Thy-1 + dendritic epidermal cell (Thy-1 +dEC) exists.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 79%

Section: Preparation Of Tissuesmentioning

confidence: 99%

Section: Staining Of Tissuesmentioning

confidence: 99%

See 2 more Smart Citations

Phenotypic Analysis of Guinea Pig Langerhans Cells with Antibodies Directed against Leucocyte Surface Antigens

Baker

Healey

Verghese

et al. 1988

Int Arch Allergy Immunol

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“…Monoclonal antibodies were screened on cryostat-sectioned material and cytospin preparations using the method of indirect immunoperoxidase described by Healey et al [1987a]. Blocking of non-specific binding sites was achieved by the addition of 50 pi of guinea pig y-globulin (10 mg/ml) to every I ml of monoclonal su pernatant and diluted second antibody.…”

Section: Immunocytocliemistrymentioning

confidence: 99%

Behaviour of Guinea Pig T Cells Stimulated by Antigen, Allo-Antigen, and Mitogen

Healey

Agha

Turk

1988

Int Arch Allergy Immunol

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The expression of major histocompatibility (MHC) antigens on guinea pig T cells was used as a functional marker for lymphocyte activation. Antigen-stimulated lymphocytes were recovered from guinea pigs responding to the contact sensitizer DNFB, and isolated T cells were then phenotyped using a new anti-guinea pig monoclonal antibody, MSgp7. The level of expression of MHC class II, as defined by the monoclonal antibody, MSgp8, was increased on T cells recovered 4 days after sensitization, as compared with unsensitized controls. The value of this experiment was extended by measuring MHC class II expression on T cells stimulated in vitro by the mitogen concanavalin A, where a clear increase in MSgp8 binding was also observed. Confirmation of the specificity of MSgp8 for guinea pig MHC class II antigens was achieved by studying the inhibitory capacity of this antibody on an MHC class II restricted mixed leucocyte reaction. The combination of antibodies MSgp7 and MSgp8 with flow cytometry could be applied to other guinea pig experimental models to quantitate the expression of MHC class II antigens on T cells to determine their putative value in disease manifestation.

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T cell proliferation induced by monoclonal antibodies to a phosphatidylinositol‐linked differentiation antigen of guinea pig lymphocytes

Schäfer

Bürger

et al. 1991

Eur J Immunol

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Monoclonal antibodies (mAb) to differentiation antigens frequently influence the in vitro function of antigen-bearing cells. We characterized a 32-36-kDa membrane protein expressed on guinea pig lymphocytes and Langerhans cells. A series of independently derived mAb to this protein, now called guinea pig T cell activation antigen (gpTAA), induced strong proliferation of T cells in vitro. Cross-linking of the mAb by a secondary antibody (rabbit anti-mouse Ig) and costimulation with phorbol 12-myristate 13-acetate were required for activation. Treatment of the cells with phosphatidylinositol-specific phospholipase C greatly reduced the amount of antigen expressed on the cell surface as measured by flow cytometry analysis. This finding indicates that the antigen is anchored to the cell membrane via phosphatidylinositol linkage as shown similarly for other membrane proteins with T cell activating properties, e.g. Thy-1 and Ly-6. The guinea pig protein differs, however, in its molecular weight and tissue distribution from similar proteins identified in the mouse or in the rat system. Unlike Thy-1, gpTAA is also expressed on B Lymphocytes and Langerhans cells. Considering the previously described involvement in cellular adhesion, and the functional characteristics reported here, gpTAA might represent a new species of differentiation antigen with T cell-activating capacity.

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An Antigenic Determinant Common to Lymphocytes and Langerhans Cells of the Guinea Pig

Cited by 9 publications

References 14 publications

Phenotypic Analysis of Guinea Pig Langerhans Cells with Antibodies Directed against Leucocyte Surface Antigens

Phenotypic Analysis of Guinea Pig Langerhans Cells with Antibodies Directed against Leucocyte Surface Antigens

Behaviour of Guinea Pig T Cells Stimulated by Antigen, Allo-Antigen, and Mitogen

T cell proliferation induced by monoclonal antibodies to a phosphatidylinositol‐linked differentiation antigen of guinea pig lymphocytes

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