The demand for more environmentally friendly alternative renewable fuels is growing as fossil fuel resources are depleting significantly. Consequently, bioethanol has attracted interest as a potentially viable fuel. The key steps in second-generation bioethanol production include pretreatment, saccharification, and fermentation. The present study employed simultaneous saccharification and fermentation (SSF) of cellulose through bacterial pathways to generate second-generation bioethanol utilizing corncobs and paper waste as lignocellulosic biomass. Mechanical and chemical pretreatments were applied to both biomasses. Then, two bacterial strains, Bacillus sp. and Norcadiopsis sp., hydrolysed the pretreated biomass and fermented it along with Achromobacter sp., which was isolated and characterized from a previous study. Bioethanol production followed by 72 h of biomass hydrolysis employing Bacillus sp. and Norcadiopsis sp., and then 72 h of fermentation using Achromobacter sp. Using solid phase micro extraction combined with GCMS the ethanol content was quantified. SSF of alkaline pretreated paper waste hydrolysed by Bacillus sp. following the fermentation by Achromobacter sp. showed the maximum ethanol percentage of 0.734±0.154. Alkaline pretreated corncobs hydrolyzed by Norcadiopsis sp. yielded the lowest ethanol percentage of 0.155±0.154. The results of the study revealed that paper waste is the preferred feedstock for generating second-generation bioethanol. To study the possible use of ethanol-diesel blends as an alternative biofuel E2, E5, E7, and E10 blend emulsions were prepared mixing commercially available diesel with ethanol. The evaluated physico-chemical characteristics of the ethanol-diesel emulsions fulfilled the Ceypetco requirements except for the flashpoint revealing that the lower ethanol-diesel blends are a promising alternative to transport fuels. As a result, the current study suggests that second generation bioethanol could be used as a renewable energy source to help alleviate the energy crisis..
Due to unsustainability of petro derived fuels, the eco-friendly energy production. As a successful alternative energy, biodiesel plays a major role to replace petrodiesel. production of biodiesel from biomasses depends on upstream processes. Lipid extraction should be effective and energy production. optimise the lipid extraction solvent system from cyanobacteria. Microcystis systems n-hexane, chloroform: methanol (2:1), n-hexane: ethanol (3:2), chloroform: n-hexane (1:1), dichloromethane: methanol (1:1), n-hexane: isopropanol (2:3) and diethyl ether for the lipid extraction method from the cyanobacterium Microcystis spp. Extracted lipid yield (g/1 g of dried biomass) and 80.09 % in each system. The gas chromatographic-mass spectrometry analysis of the products revealed that the most abundant fatty acid types of Microcystis ester (92.39 isopropanol solvent system.
Over the last decades, the negative impacts of fossil fuel on the environment and increasing demand for energy due to the unavoidable depletion of fossil fuels, has transformed the world’s interests towards alternative fuels. In particular, bioethanol production from cellulosic biomass for the transportation sector has been incrementing since the last decade. The bacterial pathway for bioethanol production is a relatively novel concept and the present study focused on the isolation of potential “cellulase-producing” bacteria from cow dung, compost soil, and termite gut and isolating sugar fermenting bacteria from palm wine. To select potential candidates for cellulase enzyme production, primary and secondary assays were conducted using the Gram’s iodine stain in Carboxy Methyl Cellulose (CMC) medium and the Dinitrosalicylic acid (DNS) assays, respectively. Durham tube assay and Solid-Phase Micro-Extraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS) was used to evaluate the sugar fermenting efficiency of the isolated bacteria. Out of 48 bacterial isolates, 27 showed cellulase activity where Nocardiopsis sp. (S-6) demonstrated the highest extracellular crude enzyme activity of endoglucanase (1.56±0.021 U) and total cellulase activity (0.93±0.012 U). The second-highest extracellular crude enzyme activity of endoglucanase (0.21±0.021 U) and total cellulase activity (0.35±0.021 U) was recorded by Bacillus sp. (T-4). Out of a total of 8 bacterial isolates, Achromobacter sp. (PW-7) was positive for sugar fermentation resulting in 3.07% of ethanol in broth medium at 48 h incubation. The results of the study revealed that Nocardiopsis sp. (S-6) had the highest cellulase enzyme activity. However, the highest ethanol percentage was achieved with by having both Bacillus sp. (T-4) and Achromobacter sp. (PW-7) for the simultaneous saccharification and fermentation (SSF) method, as compared to separate hydrolysis and fermentation (SHF) methodologies.
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