We present an approach to testing the gravitational redshift effect using the RadioAstron satellite. The experiment is based on a modification of the Gravity Probe A scheme of nonrelativistic Doppler compensation and benefits from the highly eccentric orbit and ultra-stable atomic hydrogen maser frequency standard of the RadioAstron satellite. Using the presented techniques we expect to reach an accuracy of the gravitational redshift test of order 10 −5 , a magnitude better than that of Gravity Probe A. Data processing is ongoing, our preliminary results agree with the validity of the Einstein Equivalence Principle.
Carotenogenic microalgae are unicellular photosynthetic organisms with the ability to accumulate carotenoids. Carotenoid accumulation is a protective reaction against environmental stress factors, such as bright light, extreme temperatures, etc. It makes the survival of these microorganisms under harsh environmental conditions possible. The diversity of carotenogenic microalgae has been described in detail for Central Europe and North America, as well as for tropical and subtropical latitudes with relatively favorable environmes. However, data about these microorganisms in polar and subpolar latitudes is scarce and restricted to few reports. We isolated several strains of carotenogenic microalgae from the coastal zone of the White Sea, where they were abundant. The obtained microalgae related to four species of Chlorophytes: Haematococcus lacustris, H. rubicundus, Coelastrella aeroterrestrica, and Bracteacoccus aggregatus. The last three species have been reported for polar latitudes for the first time. Most likely, carotenogenic algae in the White Sea coast are abundant due to their high physiological and metabolic plasticity, which is essential for surviving under adverse conditions of the northern regions. Pigment composition of the strains is provided. Their predominant carotenoids were astaxanthin and β-carotene. Further, the obtained strains may be considered as potential producers of natural pigments for biotechnology.
Carotenoids astaxanthin and β-carotene are widely used natural antioxidants. They are key components of functional food, cosmetics, drugs and animal feeding. They hold leader positions on the world carotenoid market. In current work, we characterize the new strain of the green microalga Bracteacoccus aggregatus BM5/15 and propose the method of its culturing in a bubble-column photobioreactor for simultaneous production of astaxanthin and β-carotene. Culture was monitored by light microscopy and pigment kinetics. Fatty acid profile was evaluated by tandem gas-chromatography–mass spectrometry. Pigments were obtained by the classical two-stage scheme of autotrophic cultivation. At the first, vegetative, stage biomass accumulation occurred. Maximum specific growth rate and culture productivity at this stage were 100–200 mg∙L−1∙day−1, and 0.33 day−1, respectively. At the second, inductive, stage carotenoid synthesis was promoted. Maximal carotenoid fraction in the biomass was 2.2–2.4%. Based on chromatography data, astaxanthin and β-carotene constituted 48 and 13% of total carotenoid mass, respectively. Possible pathways of astaxanthin synthesis are proposed based on carotenoid composition. Collectively, a new strain B. aggregatus BM5/15 is a potential biotechnological source of two natural antioxidants, astaxanthin and β-carotene. The results give the rise for further works on optimization of B. aggregatus cultivation on an industrial scale.
The severe COVID‐19 pandemic drives the research toward the SARS‐CoV‐2 virion structure and the possible therapies against it. Here, we characterized the β‐propiolactone inactivated SARS‐CoV‐2 virions using transmission electron microscopy (TEM) and atomic force microscopy (AFM). We compared the SARS‐CoV‐2 samples purified by two consecutive chromatographic procedures (size exclusion chromatography [SEC], followed by ion‐exchange chromatography [IEC]) with samples purified by ultracentrifugation. The samples prepared using SEC and IEC retained more spikes on the surface than the ones prepared using ultracentrifugation, as confirmed by TEM and AFM. TEM showed that the spike (S) proteins were in the pre‐fusion conformation. Notably, the S proteins could be recognized by specific monoclonal antibodies. Analytical TEM showed that the inactivated virions retained nucleic acid. Altogether, we demonstrated that the inactivated SARS‐CoV‐2 virions retain the structural features of native viruses and provide a prospective vaccine candidate.
We report on our efforts to test the Einstein Equivalence Principle by measuring the gravitational redshift with the VLBI spacecraft RadioAstron, in an eccentric orbit around Earth with geocentric distances as small as ∼ 7,000 km and up to 350,000 km. The spacecraft and its ground stations are each equipped with stable hydrogen maser frequency standards, and measurements of the redshifted downlink carrier frequencies were obtained at both 8.4 and 15 GHz between 2012 and 2017. Over the course of the ∼ 9 d orbit, the gravitational redshift between the spacecraft and the ground stations varies between 6.8 × 10 −10 and 0.6 × 10 −10 . Since the clock offset between the masers is difficult to estimate independently of the gravitational redshift, only the variation of the gravitational redshift is considered for this analysis. We obtain a preliminary estimate of the fractional deviation of the gravi- tational redshift from prediction of = −0.016 ± 0.003 stat ± 0.030 syst with the systematic uncertainty likely being dominated by unmodelled effects including the error in accounting for the non-relativistic Doppler shift. This result is consistent with zero within the uncertainties. For the first time, the gravitational redshift has been probed over such large distances in the vicinity of Earth. About three orders of magnitude more accurate estimates may be possible with RadioAstron using existing data from dedicated interleaved observations combining uplink and downlink modes of operation.
We consider the problem of testing the Einstein equivalence principle (EEP) by measuring the gravitational redshift with two Earth-orbiting stable atomic clocks. For a reasonably restricted class of orbits we find an optimal experiment configuration that provides for the maximum accuracy of measuring the relevant EEP violation parameter. The perigee height of such orbits is ∼1000 km and the period is 3-5 h, depending on the clock type. For the two of the current best space-qualified clocks, the VCH-1010 hydrogen maser and the PHARAO cesium fountain clock, the achievable experiment accuracy is, respectively, 1 × 10 −7 and 5 × 10 −8 after 3 years of data accumulation. This is more than 2 orders of magnitude better than achieved in Gravity Probe A and GREAT missions as well as expected for the RadioAstron gravitational redshift experiment. Using an anticipated future space-qualified clock with a performance of the current laboratory optical clocks, an accuracy of 3 × 10 −10 is reachable.
A crucial part of a space mission for very-long baseline interferometery (VLBI), which is the technique capable of providing the highest resolution images in astronomy, is orbit determination of the missions space radio telescope(s). In order to successfully detect interference fringes that result from correlation of the signals recorded by a ground-based and a space-borne radio telescope, the propagation delays experienced in the near-Earth space by radio waves emitted by the source and the relativity effects on each telescopes clock need to be evaluated, which requires accurate knowledge of position and velocity of the space radio telescope. In this paper we describe our approach to orbit determination (OD) of the RadioAstron spacecraft of the RadioAstron space-VLBI mission. Determining RadioAstrons orbit is complicated due to several factors: strong solar radiation pressure, a highly eccentric orbit, and frequent orbit perturbations caused by the attitude control system. We show that in order to maintain the OD accuracy required for processing space-VLBI observations at cm-wavelengths it is required to take into account the additional data on thruster firings, reaction wheel rotation rates, and attitude of the spacecraft.We also investigate into using the unique orbit data available only for a space-VLBI spacecraft, i.e. the residual delays and delay rates that result from VLBI data processing, as a means to evaluate the achieved OD accuracy. We present the results of the first experience of OD accuracy evaluation of this kind, using more than 5,000 residual values obtained as a result of space-VLBI observations performed over 7 years of the RadioAstron mission operations.
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