This study aimed to grade joint abnormalities in horses submitted to therapeutic arthroscopy using score-based protocols for equine joint assessment, correlated with arthroscopic treatment outcomes and owner satisfaction. In this prospective study, we evaluated 126 joints of athletic horses referred for arthroscopy. The joints were scored according to findings of medical history and physical, radiographic, ultrasonographic and arthroscopic examination. Lameness, positive response to flexion test and decreased maximum joint flexion angle were detected in more than 50% of joints. Soft tissue swelling, sclerosis, subchondral bone osteolysis and single osteochondral fragments were the most common radiographic findings. Ultrasonographic examination revealed changes in synovial fluid volume and appearance, and subchondral bone irregularities. Increased vascularity of the synovial villi, chondral fibrillation, chondral fissures and superficial cartilage erosions were the most significant arthroscopic findings. The approaches that demonstrated greater sensitivity and correlation with treatment outcome and owner satisfaction were medical history, ultrasonographic and arthroscopic examination. The scoring protocol employed to grade joint abnormalities enabled the creation of a joint score system for the objective assessment, thus determining the most frequent findings and establishing an injury severity score for each joint.
DEDICATÓRIAAos meus pais, Leiva e Hilário, por me darem a oportunidade, total apoio e força para lutar por meus objetivos e sonhos sempre, Aos meus amigos e familiares, por me ampararem em todos os momentos. AGRADECIMENTOSA Deus e Nossa Senhora Aparecida que sempre estiveram comigo e guiaram meus passos pelos caminhos que trilhei.Aos meus pais, Leiva e Hilário, que sempre me apoiaram, são meus exemplos de vida e me forneceram o que foi necessário para que conseguisse alcançar meus objetivos. Aos meus amigos e professoresÀs minhas duas casas de coração FMVZ-Unesp Botucatu e FMVZ-USP, por toda a base, inúmeros ensinamentos, profissionais e pessoais, e bons momentos vividos ao lado de pessoas especiais que conheci nessa jornada.À minha orientadora Raquel Yvonne Arantes Baccarin, que me acolheu como orientada estando sempre presente para auxiliar no projeto, nas burocracias necessárias e que mesmo sem financiamento fez com que sua realização fosse possível.À minha primeira orientadora Ana Liz Garcia Alves, que me acolheu na graduação, despertando meu interesse e paixão por pesquisa e dando a sólida base para a pós-graduação.Aos meus companheiros e irmãos de pós-graduação, Juliana, Joice, Sarah, Fernanda e Henrique que me ajudaram durante essa árdua jornada, no cuidado com nossos adoráveis árabes, nas inúmeras coletas, análises, confecção e depósito da tese.Aos meus colegas e amigos de residência e mestrado, Rodrigo, Maria Eduarda e Teresa pelo auxílio durante a realização do projeto, pelas risadas e bons momentos, deixando o fardo muito mais leve e a experiência inesquecível.Aos enfermeiros Marquinhos, Cícero, Henrique, Rosendo, Gervásio, Felipe e Ganga por toda ajuda, companheirismo e amizade nesses 5 anos de USP.Ao Giancarlo Bonagura por ceder gentilmente o gerador de ozônio para que fosse possível realizar o experimento em tempo hábil.À equipe de residentes que me auxiliou nos momentos em que precisei, além da amizade, trocas de experiência e as boas risadas.À Cristina de Oliveira Massoco Salles Gomes e Nicole, pela ajuda com a citometria de fluxo e as análises.À Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) pela concessão da bolsa de mestrado processo nº 2015/04006-9.Aos animais, nossos amados árabes, criaturas apaixonantes, que se comportaram muito durante as coletas e involuntariamente auxiliaram na realização do projeto. EPÍGRAFE "Jamais considere seus estudos como uma obrigação, mas como uma oportunidade invejável para aprender a conhecer a influência libertadora da beleza do reino do espírito, para seu próprio prazer pessoal e para proveito da comunidade à qual seu futuro trabalho pertencer" Albert Eistein Palavras-chave: Líquido sinovial. Interleucina. Sinovite. ABSTRACT VENDRUSCOLO, C. P. Evaluation of the inflammatory and oxidant effects of medical ozone in synovial joints of healthy horses. [Avaliação dos efeitos inflamatório e oxidante do ozônio medicinal em articulações sinoviais de equinos hígidos]. 2017. 92 f. Dissertação (Mestrado em Ciências) -Faculdade de Medicina Veterinária e Zoo...
ObjectiveThe aim of this study was to verify whether transient inflammatory reactions induced by intra-articular medicinal ozone administration affect joint components, by in vivo evaluation of inflammatory (prostaglandin E2, Substance P, Interleukin-6, Interleukine-1, Tumor Necrosis Factor), anti-inflammatory (Interleukin-10) and oxidative (superoxide dismutase activity and oxidative burst) biomarkers and extracellular matrix degradation products (chondroitin sulphate and hyaluronic acid) in synovial fluid.MethodsThe effects of medicinal ozone were analyzed at two ozone concentrations (groups A and B, 20 and 40 μg/ml, respectively), using oxygen-injected joints as controls (group C); each group received ten treatments (15 ml gas per treatment). Physical evaluation, evaluation of lameness, ultrasonography, and synovial fluid analysis were performed.ResultsAll joints presented mild and transient effusion throughout the study. Group B exhibited the highest lameness score on day 14 (P<0.05), detected by the lameness measurement system, probably because of the higher ozone concentration. All groups exhibited increased ultrasonography scores on day 14 (P < 0.05). Groups A and B exhibited increased proteins concentrations on day 21 (P<0.05). There was no change in hyaluronic acid concentration or the percentage of high-molecular weight hyaluronic acid throughout the experiment. Chondroitin sulfate concentrations decreased in group B, and did not change in group A and C, indicating that neither treatment provoked extracellular matrix catabolism. Cytokine and eicosanoid concentrations were not significantly changed.ConclusionsThe ozonetherapy did not cause significant inflammation process or cartilage degradation, therefore, ozonetherapy is safe at both evaluated doses.
Background Combined chondroitin sulfate (CS) and glucosamine (GlcN) has been widely used in oral formulations to prevent and treat osteoarthritis. CS is effective for controlling pain in osteoarthritic patients, whereas GlcN can stimulate glycosaminoglycan synthesis, thus reducing extracellular matrix degradation. Although several studies have been published on this topic, the effectiveness of treatment with oral CS and GlcN remains uncertain. The objective of this study was to analyze the progression of experimentally induced osteoarthritis in horses and verify the effectiveness of an oral compound based on CS and GlcN to treat and/or modulate this disease. The study analyzed the metacarpophalangeal joint of the left thoracic limb of 16 horses divided into two groups, with eight horses treated with CS and GlcN in the treated group (GT) and eight untreated horses in the control group (GC). Chondral lesions were induced through arthroscopy, which was defined as time-point zero (T0). Physical, ultrasonographic, and radiographic examinations and synovial fluid biomarkers measurements were performed on days 0, 30, 60, 90, and 120. At the end of the experiment (T4), arthroscopy was performed again to macroscopically evaluate the joints and collect material for microscopic analysis. Results Significant differences were observed between groups in some evaluated parameters, such as visual lameness assessment, synovial concentrations of prostaglandin E2, and ultrasound examination. However, the GT still presented slightly improved results for joint flexion angle, analysis of lameness using sensors, and histopathological analysis of chondral repair tissue, however, without the statistical significance (p>0.05). Conclusions The treatment was considered effective in the clinical modulation of experimental osteoarthritis, with improvement of some parameters in the GT. However, this type of treatment may not be entirely effective to change the catabolic process in articular cartilage and the progressive induced chondral damage.
Com a pandemia da Covid-19 decretada no mundo, um grupo de designers reuniu-se remotamente com especialistas de diferentes áreas e localizações geográficas, com o objetivo de desenvolver um produto inovador e confiável que ajudasse a população na prevenção da doença e no controle da dispersão da Covid-19 recente. Por meio do gerenciamento de projeto ágil e de outras ferramentas e dinâmicas já bem conhecidas no design de desenvolvimento de produto, a equipe de especialistas apresenta neste artigo o potencial e os desafios do desenvolvimento de produtos CleanUp em tempo hábil. Como principal resultado, destaca-se que a gestão ágil de projetos com equipes multidisciplinares trabalhando remotamente foi capaz de auxiliar o grupo com resultados rápidos e eficazes para o desenvolvimento do CleanUp.
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