Two novel, designated strains 29W222T and 2943T, were isolated from the marine sediment from Aoshan Bay, Jimo, PR China. Growth was observed at pH 6.0–8.5 (optimum, pH 7.5) for strain 29W222T, and pH 5.5–8.5 (pH 7.0) for strain 2943T. Both strains displayed growth in 0.5–6 % NaCl with an optimum at 1 % for 29W222T; 0.5 % for 2943T. Both strains grew optimally at 33 °C. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that 29W222T and 2943T represented members of the genus
Fulvivirga
and strain 29W222T was most closely related to
Fulvivirga kasyanovii
KMM 6220T (97.9 % sequence similarity) and
Fulvivirga imtechensis
AK7T (95.0 %), and 2943T to
Fulvivirga imtechensis
AK7T (95.7 %) and
Fulvivirga kasyanovii
KMM 6220T (94.8 %). The genomic DNA G+C contents of 29W222T and 2943T were 39.9 and 37.7 mol%, respectively. The results of chemotaxonomic analysis indicated that the sole respiratory quinone was menaquinone 7 (MK-7), and the major fatty acid was iso-C15 : 0 for both strains. Average nucleotide identity and average amino acid identity values between strain 29W222T and
Fulvivirga kasyanovii
KMM 6220T were 78.9 and 83.6 %, respectively; the corresponding values between 2943T and
Fulvivirga imtechensis
AK7T were 69.8 and 63.6 %, respectively. Therefore, strains 29W222T and 2943T represent to two novel species of the genus
Fulvivirga
, for which the names Fulvivirga marina sp. nov. (29W222T=KCTC 62848T=MCCC 1K05194T) and Fulvivirga sediminis sp. nov. (2943T=KCTC 62847T= MCCC 1K05144T) are proposed, respectively.
A new type of Ag3PO4/AgBr/hydroxyapatite photocatalyst was prepared from oyster shells, which showed high efficiency in the degradation of organic dyes.
Thermothickening polymers are a novel
type of material developed
for enhanced oil recovery applications in high-temperature and high-salinity
oil reservoirs. However, the existing synthesis process of thermoviscosifying
polymers is complex and of high cost, both of which limit the wide
application of thermoviscosifying polymers. In this study, a thermothickening
water-soluble polymer (PHAD) with a low concentration was synthesized
by a “graft from” method, with acrylamide and diacetone
acrylamide as grafted monomers and hydroxypropyl methyl cellulose
as the backbone chain. The basic parameters for PHAD copolymers were
systematically studied in comparison with their homopolyacrylamide
counterpart. The results show that the PHAD copolymers exhibited excellent
thermothickening ability even when the polymer concentration was 0.2
wt % (total salinity is 9350.08 mg·L–1) upon
increasing the temperature from 25 to 90 °C, where the apparent
viscosity enhancement changes from 4.0 to 13.3 times with increasing
the diacetone acrylamide content in PHAD copolymers. The PHAD copolymers
also showed good salt tolerance, thermal stability, and viscoelastic
properties under harsh reservoir conditions, which are attributed
to the synergistic effect of the rigid heterocyclic ring structure
and hydrophobic intermolecular association of thermoresponsive monomers
within polymer chains. Moreover, the core displacement experiment
and etched glass microscopic model show that the PHAD copolymers have
good migration in porous media. Due to its high sweep efficiency,
the PHAD copolymer has a higher recovery factor (14.0%) than polyacrylamide
(4.3%), which makes it more suitable for salt-tolerant and temperature-tolerant
tertiary oil recovery chemicals.
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