Bacterial biofilms constitute a major source of sanitary problems and economic losses in the food industry. Indeed, biofilm removal may require intense mechanical cleaning procedures or very high concentrations of disinfectants or both, which can be damaging to the environment and human health. This study assessed the efficacy of a technique based on spectroscopy in the visible, near-infrared, and short-wavelength infrared range for the quick detection of biofilms formed on polystyrene by the pathogenic bacterium Staphylococcus aureus. To do that, biofilms corresponding to three S. aureus strains, which differed in biofilm-forming ability and composition of the extracellular matrix, were allowed to develop for 5 or 24 h, representing an active formation stage and mature biofilms, respectively. Spectral analysis of the samples, corresponding to three biological replicates of each condition, was then performed by using a portable device. The results of these experiments showed that partial least-squares discriminant analysis of the spectral profile could discriminate between surfaces containing attached bacterial biomass and noninoculated ones. In this model, the two first principal components accounted for 39 and 19% of the variance and the estimated error rate stabilized after four components. Cross-validation accuracy of this assessment was 100%. This work lays the foundation for subsequent development of a spectroscopy-based protocol that allows biofilm detection on food industrial surfaces.
Spain has been pinpointed as one of the European countries at major risk of extreme urban events. Thus, Spanish cities pursue new urban plans to increase their resilience. In this scenario, experiences in the implementation of Sustainable Urban Drainage Systems (SUDS) have increased substantially. Nevertheless, few cities have developed a global urban strategy for SUDS, lacking, in many cases, a method to identify strategic areas to maximize their synergetic benefits. Furthermore, there is still a need for a holistic Multicriteria Decision Analysis (MCDA) framework that considers the four pillars of SUDS design. The city of Gijón, NW Spain, has been selected as a case study due to its environmental and climatic stresses. This research presents the methodology developed for this city, which aims to analyze the need for SUDS implementation throughout the identification of strategic areas. With this aim, a combination of Geographic Information System (GIS) software and the MCDA Analytical Hierarchical Process (AHP) were proposed. The results show the potential for SUDS’ implementation, according to nine criteria related to the SUDS’ design pillars. We found that the areas where the implementation of SUDS would bring the greatest functional, environmental and social benefits are mainly located in consolidated urban areas.
Intense urbanisation, combined with climate change impacts such as increased rainfall intensity, is overloading conventional drainage systems, increasing the number of combined sewer overflow events and making treatment plants outdated. There is a need for better urban planning, incorporating stormwater and flood management design in order to accurately design urban drainage networks. Geographic Information System (GIS) tools are capable of identifying and delineating the runoff flow direction, as well as accurately defining small-sized urban catchments using geospatial data. This study explores the synergies between GIS and stormwater management design tools for better land-use planning, providing a new methodology which has the potential to incorporate hydraulic and hydrological calculations into the design of urban areas. From data collection to final results, only freely available software and open platforms have been used: the U.S. EPA Storm Water Management Model (SWMM), QGis, PostgreSQL, PostGIS, SagaGIS, and GrassGIS. Each of these tools alone cannot provide all the necessary functionalities for large-scale projects, but once linked to GISWATER, a unique, fast, efficient, and accurate work methodology results. A case study of a newly urbanised area in the city of Gijón (northern Spain) has been utilised to apply this new methodology.
One of the usual tasks of engineers in Geomatics and Surveying is the representation of the terrain for different purposes. Within the teaching of Geomatic Engineering bachelor, the skills related to the critical assessment, contextualization, and interoperability of said terrain models require further development so that future professionals can improve their understanding of the problem, and can address day-to-day problems of fusion of data from different sources and qualities. This contribution focuses on the cartographic problem of integrating different terrain representations from various official sources and with different specifications. To this end, a teaching-learning activity based on the use of data and free software is proposed to enhance the preparation and skills of future geomatics engineering professionals, providing them with added value. The present methodology complies with the requirements of reality, economy and quality established in the scientific literature.
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