The aim of this study was to evaluate the impact of successive bovine testicular punctures using different needle sizes. Fifteen bulls were submitted to testicular needle aspiration (TNA) in the left and right testis using 18-gauge (40×12mm) or 22-gauge (25×7mm) needles, respectively, once every 30 days. Animals were randomly divided into three groups, which were submitted to bilateral orchiectomy two days after the last puncture. Group 1 (G1): only one puncture (n=5); Group 2 (G2): three consecutive punctures in a period of three months (n=5); Group 3 (G3): six consecutive punctures in a period of 6 months (n=5). Fragments from the medial portion of the testicular parenchyma were excised and fixed in Bouin's fluid for histological analysis. No differences were observed in the percentage of seminiferous tubules degeneration between G1, G2 and G3 (P>0.05). Higher amounts of erythrocyte were found in G1 and G2 groups compared to G3, in the intra-and intertubular tissue (P<0.05). There was no interaction between the needle gauge and the occurrence of testicular damage in animals submitted to one (G1) or three (G2) punctures. However, a higher percentage of tubular degeneration was associated to 18-gauge compared to 22-gauge fine needles in G3. In conclusion, multiple testicular needle aspiration can be safely conducted using fine needles. Large needles are recommended only for a single TNA, since multiple punctures may result in increased tubular degeneration and compromise testicular architecture and functionality.
Objective: The castration of male beef cattle is a routine practice in many bovine herds, but this procedure is stressful and affects profitability by reducing average of weight daily gain and increasing susceptibility to diseases. The aim of this study was to evaluate the effectiveness and security of nylon cable ties of electric usage as an alternative method for hemostasis in bovine orchiectomy. Methods: For the study, 22 animals were randomly divided into 2 groups according to the hemostasis method: Control-Group (CG, n=11) using Chromic Catgut; and Nylon Cable Ties Group (CTG, n=11). For the evaluation of the hemostasis was considered the healing time, incidence of postoperative complications and the results of hematological exams performed on days 0 (D0), 2 (D2) and 7 (D7) from the postoperative period. Results: Differences were not observed when comparing the hematocrit, total protein and plasma fibrinogen of animals castrated with nylon cable ties or Catgut in any of the evaluated moments (P>0.05). CG or CTG animals did not show alterations in any of the hematological variables serially evaluated between the moments D0 and D7 (P>0.05), except for the leukocytes count which presented significant increase, regardless of the experimental group (CG, P=0.0125; CTG, P=0.0080), event related to the transitory inflammatory process as a result of surgical procedure. Conclusion: It was concluded that the use of nylon cable ties represents an economic and effective alternative for hemostasis in bovine orchiectomy.
O objetivo do estudo foi avaliar a influência da bipartição escrotal sobre a capacidade de termorregulação do testículo caprino, testando-se a hipótese de que animais com bipartição possuem menor temperatura testicular, maior capacidade hemodinâmica local, maior número de células germinativas e maior produção espermática. Foram selecionados 8 bodes, divididos de acordo com a conformação escrotal: G1 (n=4) animais com até 50% de bipartição; GC (n=4) grupo controle, sem bipartição. Todos foram submetidos à mensuração da temperatura retal, termografia corporal e escrotal. Exames ultrassonográficos Doppler foram conduzidos no plexo pampiniforme. Adicionalmente os animais foram submetidos à punção biópsia aspirativa testicular (PBA) e 2 ejaculados de cada reprodutor foram colhidos para avaliação espermática. Não foram observadas diferenças para os resultados da PBA, da termografia escrotal e do espermograma comparando-se os dois grupos. Na avaliação hemodinâmica apenas o IR (0,53a±0,11 e 0,46b± 0,06; p=0,0108) foi superior para os animais bipartidos. Conclui-se que caprinos que possuem até 50% de bipartição escrotal possuem qualidade espermática semelhante a animais não bipartidos, não possuem menor temperatura testicular e maior número de células germinativas primordiais como se supunha na hipótese inicial da pesquisa.
ResumoOs corantes Panótico Rápido e Giemsa são frequentemente utilizados para a avaliação de células de linhagem espermatogênica, porém nenhuma destas colorações foi desenvolvida especificamente para o processamento tintorial desse tipo celular. Como até presente não existe consenso na literatura sobre qual das técnicas de coloração permite a melhor visualização das células germinativas testiculares, o objetivo deste estudo foi determinar a melhor coloração para esfregaços obtidos por punção biópsia aspirativa testicular (PBA) de bovinos. Foram selecionados 25 machos bovinos adultos, que sofreram PBAs em ambos os testículos (n=50 punções biópsia aspirativas, perfazendo um total de 100 esfregaços testiculares). O conteúdo de cada PBA foi utilizado para a confecção de no mínimo 2 esfregaços em lâminas foscas de vidro que posteriormente foram corados utilizando dois métodos distintos: Panótico Rápido (G1, n=50 esfregaços) ou Giemsa (G2, n=50). Para avaliação da qualidade dos esfregaços as células da linhagem espermatogênica foram contabilizadas e comparadas entre os dois grupos experimentais. A facilidade e nitidez de visualização proporcionada por cada uma das técnicas foi comparada atribuindo-se uma nota de 1 a 3 (1: ruim; 2: regular; 3: boa). A coloração Panótico Rápido permitiu a identificação de maior quantidade de espermatócitos secundários (p=0,0042) e células inflamatórias (p=0,0318), apresentando ainda efeito significativo sobre a qualidade de leitura dos esfregaços (p=0,0087). Embora ambas as colorações permitam a identificação e visualização das estruturas da linhagem espermatogênica, o Panótico Rápido permitiu melhor visualização e avaliação das células testiculares. Palavras Evaluation of different coloration techniques for smears obtained by aspiration biopsy puncture of bull testis Avaliação de diferentes técnicas de coloração para esfregaços obtidos por punção biópsia aspirativa testicular de bovinos AbstractThe Diff-Quick and Giemsa dye are frequently used to evaluate the germ cells, but any one was developed for the specific evaluate of this kind of cells, no existing until now, agreement in the literature about which this techniques of dye allows the best preview of the germ cells. This way, the aim of this study was determine the best dye of smears obtained with testicular aspiration biopsy puncture (TABP) of cattle. Was selected 25 males bovines adults who suffer TABP in both testicles (n=50 puncture aspiration biopsy, making a total of 100 testicular smears). The content of each TBP was used to production of at least 2 smears that later was ruddy using two different methods: Diff-Quick (n=50) or Giemsa (n=50). For the evaluate the quality of the smears the germ cells was accounted and for the determination of the best coloration techniques the cytological blades was evaluated in differential count obtained at the samples ruddy with Diff-Quick front of ruddy with Giemsa. In addition, the quality in in cellular differentiation was compared between the two groups using a scale 1 to 3 (1=bed, 2=r...
Quality of artificial insemination supplies impact the efficiency of this technique. Cover sheath model affects semen retention in artificial insemination (AI)-gun trials. Residual semen retention occurs regardless of cover sheath model used for AI. Cover sheath used in AI can influence the pregnancy rates of cows.
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