Objetivo: relatar a experiência de uso de mídias sociais para ações em saúde com idosos durante a pandemia Covid-19. Método: trata-se de um relato de experiência do projeto de extensão intitulado “Vovôs e vovós conectados: ligados na Internet na qualidade de vida”, realizado de abril a dezembro de 2020, mediado por interações síncronas e assíncronas, utilizando ferramentas digitais e discutido à luz da Política do Envelhecimento Ativo e da Década do Envelhecimento Saudável. Resultados: as abordagens virtuais basearam-se em: informações e combate às fake news sobre Covid-19; apoio emocional; promoção de saúde; e uso de tecnologia. Conclusão: evidenciou-se que o uso orientado de mídias sociais pelos idosos contribui de maneira eficaz para a manutenção das relações sociais e promoção da saúde na perspectiva do envelhecimento saudável mesmo durante uma crise sanitária de repercussão mundial.
Este trabalho teve como objetivo determinar os níveis de fator de necrose tumoral alfa (TNF-alfa) sérico e produção de peróxido de hidrogênio (H 2 O 2 ) por macrófagos peritoneais em camundongos experimentalmente infectados pelo Trypanosoma cruzi e submetidos ao treinamento físico realizado antes da infecção ou ao exercício físico durante o período de infecção. Foram utilizados camundongos BALB/c fêmeas com 30 dias de idade, inoculados com 1.400 tripomastígotas sanguíneos da cepa Y. O exercício físico consistia em atividade de intensidade moderada em esteira rolante. As dosagens foram realizadas com material coletado no 13º dia de infecção. Para a dosagem de TNF-alfa, foi utilizada a técnica de ELISA de captura. A produção de H 2 O 2 foi expressa por coloração produzida após a incubação de macrófagos peritoneais com peroxidase e a leitura realizada em aparelho de ELISA. Não foram encontradas diferenças significativas nas concentrações de TNF-alfa e produção de H 2 O 2 entre os grupos infectados. O treinamento físico realizado antes da infecção e o exercício físico realizado após a infecção não foram capazes de alterar significativamente os níveis de TNF-alfa e a produção de H 2 O 2 na infecção pelo T. cruzi.Palavras-chave: exercício físico, Trypanosoma cruzi, TNF-alfa, H 2 O 2 . ABSTRACT. Production of tumoral necrosis factor-alpha and hydrogen peroxide duringTrypanosoma cruzi infection in mice submitted to exercise. This study aimed to determine the levels of seric tumor necrosis factor-alpha (TNF-alpha) and production of hydrogen peroxide (H 2 O 2 ) by peritoneal macrophages in mice experimentally infected with T. cruzi and submitted to pre-infection exercise training and to post-infection acute exercise. Female 30-day-old BALB/c mice were inoculated with 1,400 blood trypomastigotes of Ystrain T. cruzi. Exercise programs consisted in moderate-intensity activity and were carried out in a treadmill. The measurements were performed with material collected at the 13 th day after infection Serum TNF-alpha was evaluated using capture ELISA. H 2 O 2 production was expressed by coloration produced after incubation of peritoneal macrophages and the measurement was performed using an ELISA reader. There were no statistically significant differences in TNF-alpha levels and H 2 O 2 production between the trained and non-trained infected groups. Thus, the physical training performed before infection and physical exercise performed after the infection were not able to change the levels of TNF-alpha and production of H 2 O 2 in the infection by T. cruzi.
Background: Chagas disease is an infection caused by Trypanosoma cruzi that affects eight million people in Latin America. One factor linked to the lifestyle that significantly interferes in the response to infection is physical exercise, depending on the kind, intensity and frequency of the activity practiced. Objective: To evaluate the influence of pre-infection chronic moderate aerobic exercise in the development of experimental infection with T. cruzi in mice of two distinct lineages from both sexes. Methods: 30-day old Swiss and BALB/c mice (male and female) were divided into four groups for each strain and sex (total 16) and named as follows: SM (Swiss males), SF (Swiss females) BM (BALB/c mice) and BF (BALB/c mice). The groups were: NT NI (untrained uninfected) T NI (trained not infected); NT I (untrained infected), TI (trained infected). The aerobic exercise pre-moderate chronic infection training was performed with one daily session for eight weeks, five times a week. The inoculum was 1,400 blood trypomastigotes of Y strain of T. cruziintraperitoneally. The peak of parasites, parasitemia total and average measurements of the serum activities of CK and CK-MB were evaluated. Results and Conclusions: The physical training promoted reduction in peak, parasitemia parasites and total average in animals infected with T. cruzi in both strains and sexes. Physical training induced reduction in serum activities of CK and CK-MB in animals infected with T. cruzi of both sexes and from the two strains, except for females in the Swiss CK activity.
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