Abstract-The effective design of field studies requires that sample size requirements be estimated for important endpoints before conducting assessments. This a priori calculation of sample size requires initial estimates for the variability of the endpoints of interest, decisions regarding significance levels and the power desired, and identification of an effect size to be detected. Although many programs have called for use of critical effect sizes (CES) in the design of monitoring programs, few attempts have been made to define them. This paper reviews approaches that have been or could be used to set specific CES. The ideal method for setting CES would be to define the level of protection that prevents ecologically relevant impacts and to set a warning level of change that would be more sensitive than that CES level to provide a margin of safety; however, few examples of this approach being applied exist. Program-specific CES could be developed through the use of numbers based on regulatory or detection limits, a number defined through stakeholder negotiation, estimates of the ranges of reference data, or calculation from the distribution of data using frequency plots or multivariate techniques. The CES that have been defined often are consistent with a CES of approximately 25%, or two standard deviations, for many biological or ecological monitoring endpoints, and this value appears to be reasonable for use in a wide variety of monitoring programs and with a wide variety of endpoints.
Previous research about the biogeochemistry of beaver (Castor canadensis Kuhl) impoundments has generally overlooked the influence of soil type on solutes. In this study, soils derived from glacio‐lacustrine and glacio‐fluvial parent materials in northern Minnesota beaver meadows (i.e., former beaver ponds that have drained and revegetated) were studied to: (i) describe soil morphology, (ii) analyze soil and solute chemistry, and (iii) statistically evaluate the relative influence of hydrology and soil type on solute chemistry. With decreasing depth to groundwater, soils in both hydrosequences were increasingly reducing; redox potentials were consistently negative in the Borosaprist. Differences in soil morphology associated with increasing wetness included: (i) increasing thickness of O and A horizons, (ii) decreasing thickness of the Bt horizon (glacio‐lacustrine hydrosequence), (iii) disappearance of E and Bk horizons (glacio‐lacustrine hydrosequence), and (iv) decreasing depth to redoximorphic features. In the glacio‐lacustrine hydrosequence, depth to subsurface concentrations of oxalateextractable Fe (≥800 g m−3) decreased with increasing wetness. Two‐way ANOVA indicated that differences in water chemistry among soils were due to moisture, parent material, and their interaction. Increasing moisture was associated with increased concentrations of Fe2+, Ca2+, and Mg2+ and decreased concentrations of SO4‐S. Glaciolacustrine soils contained higher concentrations of base cations (K+, Ca2+, and Mg2+) than did glacio‐fluvial soils, and so did their soil water. There were pronounced seasonal variations in cation concentrations and significant interannual differences for NH4‐N, total N, and K+. Nitrate concentrations were consistently low and did not differ significantly among any of the groupings.
Oil sands-influenced process waters have been observed to cause reproductive effects and to induced CYP1A activity in fishes; however, little progress has been made in determining causative agents. Naphthenic acids (NAs) are the predominant organic compounds in process-affected waters, but due to the complexity of the mixture, it has been difficult to examine causal linkages in fishes. The aim of this study was to use in vitro assays specific to reproductive and CYP1A mechanisms to determine if specific acid extractable fractions of NAs obtained from oil sands-influenced water are active toward reproductive processes or interact with the Ah receptor responsible for CYP1A activity. NAs were extracted from aged oil sands-influenced waters by use of acid precipitation, and the mixture was fractionated into three acidic and one neutral fraction. The four fractions were examined for Ah receptor-mediated potency by use of the H4IIE-luc bioassay, effects on production of steroid hormones by use of the H295R steroidogenesis assay, and sex steroid receptor binding activity using the yeast estrogen screen and yeast androgen screen. The mixtures were characterized by high resolution mass spectrometry, (1)H nuclear magnetic resonance, and attenuated total reflectance infrared spectroscopy. The neutral fraction elicited Ah-receptor mediated activity after 24 h but not after 48 or 72 h. None of the fractions contained measurable levels of estrogen or androgen receptor agonists nor did they cause reductions in steroidogenesis. A number of fractions showed antiestrogenic or antiandrogenicity potency, with the neutral and main acidic fractions being the most potent. Neutral aromatic compounds are likely responsible for the CYP1A activity observed. Direct estrogenic, androgenic, or steroidogenic mechanisms are unlikely for NAs based on these results, but NAs act as potent antiandrogen or antiestrogens.
To investigate impacts of proposed oil sands aquatic reclamation techniques on benthic fish, white sucker (Catostomus commersonii Lacépède, 1803) were stocked in 2 experimental ponds-Demonstration Pond, containing aged fine tailings capped with fresh water, consistent with proposed end-pit lake designs, and South Bison Pond, containing aged unextracted oil sands material-to examine the effects of unmodified hydrocarbons. White sucker were stocked from a nearby reservoir at both sites in May 2010 and sampled 4 mo later to measure indicators of energy storage and utilization. Comparisons were then made with the source population and 2 reference lakes in the region. After exposure to aged tailings, white sucker had smaller testes and ovaries and reduced growth compared with the source population. Fish introduced to aged unextracted oil sands material showed an increase in growth over the same period. Limited available energy, endocrine disruption, and chronic stress likely contributed to the effects observed, corresponding to elevated concentrations of naphthenic acids, aromatic compounds in bile, and increased CYP1A activity. Because of the chemical and biological complexity of these systems, direct cause-effect relationships could not be identified; however, effects were associated with naphthenic acids, polycyclic aromatic hydrocarbons, ammonia, and high pH. Impacts on growth have not been previously observed in pelagic fishes examined in these systems, and may be related to differences in sediment interaction.
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