Testosterone, Androst-4-en-3,17-dione, Enzyme Induction, S trep to m yces hydrogenans After cultivation of S trep to m yces h ydrogenan s in the presence of 3H-labelled testosterone, radio active steroids were extracted separately from the cytosolic, ribosomal and cell wall-membrane fraction of the cells and from the culture medium, respectively.. The separation of the steroids was performed by one-and two-dimensional thin layer chromatography (TLC). The identification of the main metabolites was achieved by crystallization to constant specific radioactivity, specific staining procedures and acetylation. The oxidation of testosterone to androst-4-en-3,17-dione is by far the predominating reaction, which is almost finished after 3 h cultivation. Androst-4-en-3,17-dione is mainly transferred into the culture medium and partly accumulated within the cell wallmembrane fraction. High polar steroid metabolites and androstane derivatives are present in very small amounts only.
Multiple colorectal adenocarcinomas were found in Sprague-Dawley rats after intrarectal injection of 1,2-dimethylhydrazine (DMH). These lesions along with mild hyperplasia of the surrounding mucosa developed within 34 weeks after either 3 or 8 injections of 25 0 mg DMH/kg body weight.
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