A group of 53 normal human plasma samples showed variable staphylococcal anti-delta-hemolysin activity. Albumin and globulin in low concentrations did not interfere with the hemolytic activity of delta lysin. Rabbit and guinea pig sera contained anti-delta lysin in low titer. Staphylococcus aureus 209 produced equal zones of hemolysis when grown on washed erythrocyte agar plates prepared from 16 different human blood samples while whole blood agar plates prepared from the same blood gave variable and smaller zones under similar conditions.
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