Prevalence of Staphylococcal Anti-Delta Lysin in Man

Maniar, A. C.; Weslake, Claudia; Warner, Peter

doi:10.1139/m67-124

Cited by 8 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The occurrence of a factor in serum that neutralizes delta hemolysin was initially observed by Marks and Vaughan (1950) and confirmed by Jackson and Little (1958). Maniar, Weslake, and Warner (1967) observed that all samples of human plasma tested had an inhibitory effect on delta hemolysin. An attempt was made d~~r i n g the present study to establish the quantity of delta hemolysin inhibitor in humail sera.…”

mentioning

confidence: 77%

Antistaphylococcal hemolysins and delta hemolysin inhibitor in adult human serum

Donahue¹

1969

Can. J. Microbiol.

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 77%

Antistaphylococcal hemolysins and delta hemolysin inhibitor in adult human serum

Donahue¹

1969

Can. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Nonimmune sera from a variety of species precipitate delta-toxin in agar gels and neutralize its hemolytic activity. These effects have been considered by some investigators to be due to the antibody (15), whereas other investigators view them as reflecting an interaction of toxin with lipoproteins of phospholipids (3,10,12). By immunoelectrophoretic analyses, it was possible to examine preimmune whole sera, without prior fractionation, for the presence of delta-toxinreactive IgG.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of Staphylococcus aureus delta-toxin

Nolte¹,

Kapral²

1981

Infect Immun

View full text Add to dashboard Cite

Studies were conducted to determine the immunogenicity of purified Staphylococcus aureus delta-toxin. Rabbits and guinea pigs immunized with delta-toxin incorporated into a multiple emulsion consisting of complete Freund adjuvant with 1% Tween 80 produced antibody, whereas animals given toxin in saline or toxin in saline with Tween 80 did not produce antibody. The immunoglobulin G (IgG) fraction isolated by chromatography on protein A-Sepharose was examined for the presence of anti-delta-toxin antibody by immunoelectrophoresis, immunodiffusion, quantitative precipitation tests, affinity chromatography, and toxin neutralization tests. Although delta-toxin-specific IgG precipitated the toxin in agar gels, the antibody did not neutralize the toxin's hemolytic activity. Deltatoxin binding to human erythrocyte membranes was demonstrated by indirect immunofluorescent staining of toxin-treated erythrocytes.There have been conflicting reports concerning the immunogenicity of Staphylococcus aureus delta-toxin. Gladstone and Yosida (7), Hallander (8), and Kantor et al. (10) were unable to produce antibody against purified delta-toxin. More recently, Fackrell and Wiseman (4), Heatley (9), Birkbeck and Whitelaw (1), and Turner (18) reported the production of antidelta-toxin immunoglobulin in response to immunization of rabbits with milligram quantities of the toxin.Many investigators have shown that normal sera from a variety of species not only neutralize the hemolytic activity of the toxin, but also form precipitin lines with the toxin in agar gels (3,7,9,10). Whitelaw and Birkbeck (21) have shown that both a and / lipoproteins of normal human serum inhibit the action of delta-toxin. Kapral observed that phospholipids and certain longchain fatty acids inhibit delta-toxin and suggested that such lipids might be responsible for the neutralizing activity of normal serum (11,12). Although the presence of nonspecific inhibitors in normal serum must be recognized, they do not pertain to the question of the immunogenicity of delta-toxin.In this study, immunoglobulin G (IgG) isolated from preimmune and immune sera was examined for the presence of anti-delta-toxin antibody by immunoelectrophoresis, immunodiffusion, quantitative precipitation tests, affinity chromatography, and toxin neutralization tests. Delta-toxin-specific IgG was produced in rabbits and guinea pigs in response to immunization with delta-toxin in complete Freund adjuvant, but the antibodies did not neutralize the t Present address:

show abstract

“…There has also been controversy concerning the antigenicity of the toxin. Several investigators were unable to raise antibody against purified delta-toxin (60,64,78) in spite of the fact that serum can neutralize delta-toxin activity (39,60,109). However, data by Donahue (39) and Kapral (79) indicated that either serum lipoproteins or serum phospholipids, and not immunoglobulin, caused this neutralization.…”

Section: Propertiesmentioning

confidence: 99%