BackgroundThe desulphurization of dibenzothiophene (DBT), a recalcitrant thiophenic fossil fuel component by Serratia marcescens (UCP 1549) in order for reducing the Sulphur content was investigated. The Study was carried out establishing the growth profile using Luria Bertani medium to different concentrations of DBT during 120 hours at 28°C, and orbital Shaker at 150 rpm.ResultsThe results indicated that concentrations of DBT 0.5, 1.0 and 2.0 mM do not affected the growth of the bacterium. The DBT showed similar Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MCB) (3.68 mM). The desulphurization of DBT by S. marcescens was used with 96 hours of growth on 2 mM of DBT, and was determined by gas chromatography (GC) and GC-mass spectrometry. In order to study the desulphurization process by S. marcescens was observed the presence of a sulfur-free product at 16 hours of cultivation.ConclusionsThe data suggests the use of metabolic pathway “4S” by S. marcescens (UCP 1549) and formed biphenyl. The microbial desulphurization process by Serratia can be suggest significant reducing sulphur content in DBT, and showed promising potential for reduction of the sulfur content in diesel oil.
<p align="justify">Realizou-se estudos avaliando a ação da biomassa viva e inativada de Cunninghamella elegans crescida, visando à remoção do hidrocarboneto aromático policíclico fenantreno. Monitorou-se o processo de remoção do fenantreno a cada 24 h por espectrofotometria, de acordo com um planejamento fatorial completo de 22 e, como variáveis independentes 0,1 e 0,2 mM de fenantreno, na presença de 0,6, 3,3 e 6% de glicose e variável resposta o conteúdo de fenantreno removido. Os resultados obtidos indicaram que tanto a biomassa inativada como viva removeram, respectivamente, 90,0% e 82,0% a concentração de 0,2mM de fenantreno na presença da menor concentração de glicose (0,6%). A biomassa inativada de C. elegans demonstrou ser um sorbente com elevado potencial biotecnológico para a remediação de áreas poluídas com hidrocarbonetos aromáticos policíclicos.</p><p align="justify">Abstract</p><p align="justify">Studies were carried out evaluating the action of the life and inactivated biomass of Cunninghamella elegans grown to removal polycyclic aromatic hydrocarbon phenanthrene. The removal process was monitored each 24h by spectrophotometry, de acordo com according to full factorial design 2 2 , and independent variables the phenanthrene concentrations [0.1 and 0.2 mM], and the content of phenanthrene removed as variable response.The results showed that both inactivated and live biomass from C. elegans removed 85.0% and 90.0%, respectively, of phenantrene of 0.2 mM on glucose (0.6) concentration. The inactivated biomass from C. elegans shows a sorbent with high biotechnological potential for bioremediation of polluted area with polycyclic aromatic hydrocarbons.</p>
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