Oxidation of dibenzothiophene (DBT) by Serratia marcescens UCP 1549 formed biphenyl as final product

Araújo, Hélvia Walewska Casullo de; Siva, M.; Lins, Clarissa Isabel Matos; Nascimento, Aline Elesbão do; Silva, Carlos Alberto Alves da; Campos-Takaki, Galba Maria de

doi:10.1186/1754-6834-5-33

Cited by 20 publications

(9 citation statements)

References 49 publications

(74 reference statements)

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“…The released sulphur may be assimilated inside the microbial cells to sustain their growth (de Araújo et al . 2012). This may attribute to the bacterial cells consuming the produced sulphate ion in order to satisfy their growth requirements (Chandra et al .…”

Section: Discussionmentioning

confidence: 99%

Desulphurisation kinetics of thiophenic compound by sulphur oxidizing Klebsiella oxytoca SOB‐1

et al. 2020

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Aims The major aims of this study are to determine the capability of sulphur oxidizing bacterium (SOB‐1) to desulphurize dibenzothiophene (DBT) and crude oil, detection of the reaction kinetics and identify the proposed pathway of DBT desulphurization. Methods and Results The isolate was genetically identified based on 16S rRNA gene sequencing as Klebsiella oxytoca and deposited in the Genebank database under the accession number: MT355440. The HPLC analysis of the remaining DBT concentration revealed that, SOB‐1 could desulphurize 90% of DBT (0·25 mmol l−1) within 96 h. The maximum production of sulphate ions from the desulphurization of DBT (0·36 mmol l−1) and crude oil (0·4 mmol l−1) could be quantitatively detected after 48 h of incubation at 30°C. The high values of correlation coefficient (R2) obtained at all studied concentrations; suggested that biodesulfurization kinetics of DBT follows the first‐order reaction model. The kinetics studies showed that, DBT may have an inhibitory effect on SOB‐1 when the initial concentration exceeded 0·75 mmol l−1. The GC‐MS analysis exhibited four main metabolites rather than DBT. The most important ones are 2‐hydroxybiphenyl (2‐HBP) and methoxybiphenyl n(2‐MBP). Conclusions Klebsiella oxytoca SOB‐1 catalyzes the desulphurization of DBT through 4S pathway and forms four main metabolic products. The release of sulphate ion and formation of 2‐HBP indicating the elimination of sulphur group without altering the carbon skeleton of DBT. The bacterial strain could also catalyzes desulphurization of crude oil. The desulphurization kinetics follows the first‐order reaction model. Significance and Impact of the Study Klebsiella oxytoca SOB‐1 could be used as a promising industrial and environmental biodesulfurizing agent as it is not affecting carbon skeleton of thiophenic compounds and forming less toxic metabolic product (2‐MBP).

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Section: Discussionmentioning

confidence: 99%

Desulphurisation kinetics of thiophenic compound by sulphur oxidizing Klebsiella oxytoca SOB‐1

et al. 2020

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“…Bacteria following 4S pathway of biocatalytic desulfurization release the S atom from organosulfur compounds via C-S oxidative bond cleavage. This sulfur is assimilated mainly in the form of sulfur containing amino acids to sustain the microbial growth [ 44 ]. Nevertheless, in 4S pathway the carbon frame of thiophenic compounds is not degraded and its major advantage is calorific value of the fuel after desulfurization is unaltered.…”

Section: Discussionmentioning

confidence: 99%

Proteomics and Metabolomics Analyses to Elucidate the Desulfurization Pathway of Chelatococcus sp.

et al. 2016

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Desulfurization of dibenzothiophene (DBT) and alkylated DBT derivatives present in transport fuel through specific cleavage of carbon-sulfur (C-S) bonds by a newly isolated bacterium Chelatococcus sp. is reported for the first time. Gas chromatography-mass spectrometry (GC-MS) analysis of the products of DBT degradation by Chelatococcus sp. showed the transient formation of 2-hydroxybiphenyl (2-HBP) which was subsequently converted to 2-methoxybiphenyl (2-MBP) by methylation at the hydroxyl group of 2-HBP. The relative ratio of 2-HBP and 2-MBP formed after 96 h of bacterial growth was determined at 4:1 suggesting partial conversion of 2-HBP or rapid degradation of 2-MBP. Nevertheless, the enzyme involved in this conversion process remains to be identified. This production of 2-MBP rather than 2-HBP from DBT desulfurization has a significant metabolic advantage for enhancing the growth and sulfur utilization from DBT by Chelatococcus sp. and it also reduces the environmental pollution by 2-HBP. Furthermore, desulfurization of DBT derivatives such as 4-M-DBT and 4, 6-DM-DBT by Chelatococcus sp. resulted in formation of 2-hydroxy-3-methyl-biphenyl and 2-hydroxy –3, 3/- dimethyl-biphenyl, respectively as end product. The GC and X-ray fluorescence studies revealed that Chelatococcus sp. after 24 h of treatment at 37°C reduced the total sulfur content of diesel fuel by 12% by per gram resting cells, without compromising the quality of fuel. The LC-MS/MS analysis of tryptic digested intracellular proteins of Chelatococcus sp. when grown in DBT demonstrated the biosynthesis of 4S pathway desulfurizing enzymes viz. monoxygenases (DszC, DszA), desulfinase (DszB), and an NADH-dependent flavin reductase (DszD). Besides, several other intracellular proteins of Chelatococcus sp. having diverse biological functions were also identified by LC-MS/MS analysis. Many of these enzymes are directly involved with desulfurization process whereas the other enzymes/proteins support growth of bacteria at an expense of DBT. These combined results suggest that Chelatococcus sp. prefers sulfur-specific extended 4S pathway for deep-desulphurization which may have an advantage for its intended future application as a promising biodesulfurizing agent.

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“…The ability of microorganisms to use DBT as the sole source of sulfur helps to remove excess sulfur from crude oil and products of its refining. The presence of DBT desulfurization pathways has been found in microorganisms of various genera [ [9] , [10] , [11] ]. The discovery of a highly specific 4S pathway (sulfur-specific pathway) in Rhodococcus erythropolis IGTS8 (ATCC 53968) isolated in 1990 at the Gas Technology Institute (USA) is of great importance for the study of microbial desulfurization of fossil fuels.…”

Section: Introductionmentioning

confidence: 99%

Characterization and genomic analysis of Gordonia alkanivorans 135, a promising dibenzothiophene-degrading strain

Delegan

Kocharovskaya

Frantsuzova

et al. 2021

Biotechnology Reports

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Oxidation of dibenzothiophene (DBT) by Serratia marcescens UCP 1549 formed biphenyl as final product

Cited by 20 publications

References 49 publications

Desulphurisation kinetics of thiophenic compound by sulphur oxidizing Klebsiella oxytoca SOB‐1

Desulphurisation kinetics of thiophenic compound by sulphur oxidizing Klebsiella oxytoca SOB‐1

Proteomics and Metabolomics Analyses to Elucidate the Desulfurization Pathway of Chelatococcus sp.

Characterization and genomic analysis of Gordonia alkanivorans 135, a promising dibenzothiophene-degrading strain

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