Objective: To verify presence and severity of muscular and/or intravascular damage during a subterranean exploration of long duration. Methods: We measured serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) as markers of muscular damage. We also measured haptoglobin as a marker of intravascular haemolysis, and platelets and leucocytes as markers of inflammation. Results: We found in all the participants an increase in CK, LDH, and platelets and leucocytes (mainly due to neutrophilia and monocytosis), and a decrease in the level of haptoglobin and circulating lymphocytes. Conclusions: The observed data suggest that continuous effort during long alpine subterranean explorations, environmental conditions, sleep deprivation, multiple impacts on rocks, and compression caused by bindings of the caving harness cause muscle damage, intravascular haemolysis, inflammation response, and immunological changes.

No abstract

Five eosinophil peroxidase (EPO)-deficient subjects were identified from 131,000 peripheral blood samples examined for routine automated analysis. The EPO-deficient eosinophils of these subjects met the main criteria established for EPO deficiency: absent or strongly decreased reaction for peroxidase, absent or strongly decreased staining with Sudan Black, and an increased ratio of the granule core volume to the total granule volume. In this report we show that this granule alteration is caused mainly by a decrease of its volume, particularly of the matrix, and that two other matrix proteins, eosinophil cationic protein and eosinophil derived neurotoxin, appear to be present in normal amounts in the EPO-deficient granules.

We studied the hypothalamus-pituitary adrenocortical, hypothalamus-pituitary and hypothalamus-pituitary thyroid system responses to a long duration activity (about 20 h) practiced in a demanding environment, characterized by darkness, low temperature and high humidity, namely alpine potholing. We performed four blood drawings in five elite potholers: (1) the morning before the performance, (2) at the bottom of the cave (-700 m), (3) at the end of the ascent, and (4) after 24 h of recovery. Two blood drawings as controls were performed on the same potholers, at the same resting time and with the same experimental procedures as the previous ones. Friedman two-way ANOVA test evidenced significant changes through the different time intervals for detrended (i.e., test values minus control values) growth hormone (GH) (P = 0.003), detrended cortisol (P = 0.004) and FT4 (P = 0.002), while this was not true for TSH and FT3. Successively pairwise comparisons were done both through the different time intervals and between test and control values. The rise of GH values during the performance underlines the great intensity and long duration characteristic of potholing as well as the possibility that the climbing sit harness can cause problems due to vascular hypo-perfusion. Cortisol data, peaking before entering the cave, suggest that there was a marked anticipatory stress reaction followed by less stressing phase during the performance. Finally, the rise of FT4 is likely due to the typical increase of free fatty acids that usually occurs during endurance exercise.

These preliminary findings show that scuba diving and dry hyperbaric exposure, although at the same environmental pressure, cause different effects on ventricular loads in healthy subjects.