Honeybee (Apis mellifera L.) is a model organism, contributing signi cant effect on global ecology by pollination and examining due to its social behaviour. MethodsIn this study, barley-speci c Sukkula and Nikita retrotransposons were analysed using IRAP (Inter-Retrotransposon Ampli cation Polymorphism) marker technique, and the relationships between retrotransposon movements and development were also investigated in three different colonies of the Caucasian bee (Apis mellifera caucasica). Furthermore, transposon sequences belonging to Apis mellifera, Bombus terrestris, Triticum turgidum and Hordeum vulgare were also examined to gure out evolutionary relationships. ResultsFor this purpose, a queen bee, ve worker bees, and ve larvae from each colony were studied. Both retrotransposons were found in all samples in three colonies with different polymorphism ratios (0-100% for Nikita and 0-67% for Sukkula). We also determined polymorphisms in queen-worker (0-83% for Nikita, 0-63% for Sukkula), queen-larvae (0-83% for Nikita, 0-43% for Sukkula) and worker-larvae comparisons (0-100% for Nikita, 0-63% for Sukkula) in colonies. Moreover, close relationships among transposons found in plant and insect genomes as a result of in silico evaluations to verify experimental results. ConclusionThis work could be one of the rst studies to analyse plant-speci c retrotransposons' movements in honeybee genome. Results are expected to understand evolutionary relationships in terms of horizontal transfer of transposons among kingdoms.
In this study, retrotransposons mobility in eight pure lines of the domestic chicken (Gallus gallus domesticus) was analysed using IRAP-PCR (Inter-retrotransposon amplification polymorphism Polymerase Chain Reaction) method. Polymorphism rates were detected as 0–78% in Copia-like, 0–73% in Nikita, 0–60% in SIRE, and 0–38 in Sukkula in Rhode Island Red, Barred Rock, Colombian Rock, Line-54, Black Line, Blue Line, Brown Line, and Maroon Line chickens. In silico analyses presented that Blue Line with Line-54 and Barred Rock with Colombian Rock are closely related regarding these four retrotransposons. These horizontally transferred plant-specific retrotransposons may affect changes in the pure-line chicken genome.
Objective Honeybee (Apis mellifera L.) is a model organism, contributing significant effect on global ecology by pollination and examining due to its social behaviour. Methods In this study, barley-specific Sukkula and Nikita retrotransposons were analysed using IRAP (Inter-Retrotransposon Amplification Polymorphism) marker technique, and the relationships between retrotransposon movements and development were also investigated in three different colonies of the Caucasian bee (Apis mellifera caucasica). Furthermore, transposon sequences belonging to Apis mellifera, Bombus terrestris, Triticum turgidum and Hordeum vulgare were also examined to figure out evolutionary relationships. Results For this purpose, a queen bee, five worker bees, and five larvae from each colony were studied. Both retrotransposons were found in all samples in three colonies with different polymorphism ratios (0-100% for Nikita and 0-67% for Sukkula). We also determined polymorphisms in queen-worker (0-83% for Nikita, 0-63% for Sukkula), queen-larvae (0-83% for Nikita, 0-43% for Sukkula) and worker-larvae comparisons (0-100% for Nikita, 0-63% for Sukkula) in colonies. Moreover, close relationships among transposons found in plant and insect genomes as a result of in silico evaluations to verify experimental results. Conclusion This work could be one of the first studies to analyse plant-specific retrotransposons’ movements in honeybee genome. Results are expected to understand evolutionary relationships in terms of horizontal transfer of transposons among kingdoms.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.