A method to prepare uniform styrene/maleimide copolymer (SMI) microspheres from poly(styrene-alt-maleic anhydride) (SMA) and gaseous amine by solvent-free pathway was developed. In this approach, SMA microspheres were firstly prepared by...
Immobilization
of protein at high efficiency is a challenge for
fabricating polymer-based protein chips. Here, a simple but effective
approach was developed to fabricate a cyclic olefin copolymer (COC)-based
protein microarray with a high immobilization density. In this strategy,
poly(maleic anhydride-co-vinyl acetate) (poly(MAH-co-VAc)) brushes were facilely attached on the COC surface
via UV-induced graft copolymerization. The introduction of poly(MAH-co-VAc) brushes resulted in an obvious increase in the surface
roughness of COC. The functionalized COC showed little reduction in
transparency compared with pristine COC, indicating that the photografting
treatment did not alter its optical property. The graft density of
the anhydride groups on the modified COC could be tuned from 0.46
to 3.2 μmol/cm2. The immobilization efficiency of
immunoglobulin G (IgG) on functionalized COC reached 88% due to the
high reactivity between anhydride groups and amine groups of IgGs.
An immunoassay experiment demonstrated that the microarray showed
high sensitivity to the target analyte.
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