Realistic two-dimensional (2D) and three-dimensional (3D) applications for anatomical studies are being developed from true-colored sectioned images. We generated advanced-sectioned images of the entire male body and verified that anatomical structures of both normal and abnormal shapes could be visualized in them. The cadaver was serially sectioned at constant intervals using a cryomacrotome. The sectioned surfaces were photographed using a digital camera to generate horizontal advanced-sectioned images in which normal and abnormal structures were classified. Advanced-sectioned images of the entire male body were generated. The image resolution was 3.3  3.3 fold better than that of the first sectioned images obtained in 2002. In the advancedsectioned images, normal and abnormal structures ranging from microscopic (≥0.06 mm  0.06 mm; pixel size) to macroscopic (≤473.1 mm  202 mm; body size) could be identified. Furthermore, the real shapes and actual sites of lung cancer and lymph node enlargement were ascertained in them. Such images will be useful because of their true color and high resolution in digital 2D and 3D applications for gross anatomy and clinical anatomy. In future, we plan to generate new advanced-sectioned images of abnormal cadavers with different diseases for clinical anatomy studies.

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Background:To properly utilize the sectioned images in a Visible Monkey dataset, it is essential to segment the images into distinct structures. This segmentation allows the sectioned images to be compiled into two-dimensional or three-dimensional software packages to facilitate anatomy and radiology education, and allows them to be used in experiments involving electromagnetic radiation. The purpose of the present study was to demonstrate the potential of the sectioned images using the segmented images. Methods: Using sectioned images of a monkey's entire body, 167 structures were segmented using Adobe Photoshop. The segmented images and sectioned images were packaged into the browsing software. Surface models were made from the segmented images using Mimics. Volume models were made from the sectioned images and segmented images using MRIcroGL. Results: In total, 839 segmented images of 167 structures in the entire body of a monkey were produced at 0.5-mm intervals (pixel size, 0.024 mm; resolution, 8,688 × 5,792; color depth, 24-bit color; BMP format). Using the browsing software, the sectioned images and segmented images were able to be observed continuously and magnified along with the names of the structures. The surface models of PDF file were able to be handled freely using Adobe Reader. In the surface models, the space information of all segmented structures was able to be identified using Sim4Life. On MRIcroGL, the volume model was able to be browsed and sectioned at any angle with real color. Conclusion: Browsing software, surface models, and volume models are able to be produced based on the segmentation of the sectioned images. These will be helpful for students and researchers studying monkey anatomy and radiology, as well as for biophysicists examining the effects of electromagnetic radiation.

When performing deep brain stimulation (DBS) of the subthalamic nucleus, practitioners should interpret the magnetic resonance images (MRI) correctly so they can place the DBS electrode accurately at the target without damaging the other structures. The aim of this study is to provide a real color volume model of a cadaver head that would help medical students and practitioners to better understand the sectional anatomy of DBS surgery. Sectioned images of a cadaver head were reconstructed into a real color volume model with a voxel size of 0.5 mm × 0.5 mm × 0.5 mm. According to preoperative MRIs and postoperative computed tomographys (CT) of 31 patients, a virtual DBS electrode was rendered on the volume model of a cadaver. The volume model was sectioned at the classical and oblique planes to produce real color images. In addition, segmented images of a cadaver head were formed into volume models. On the classical and oblique planes, the anatomical structures around the course of the DBS electrode were identified. The entry point, waypoint, target point, and nearby structures where the DBS electrode could be misplaced were also elucidated. The oblique planes could be understood concretely by comparing the volume model of the sectioned images with that of the segmented images. The real color and high resolution of the volume model enabled observations of minute structures even on the oblique planes. The volume models can be downloaded by users to be correlated with other patients’ data for grasping the anatomical orientation.

BACKGROUND: Knowledge regarding the hypothalamic nuclei is essential for understanding neuroanatomy and has substantial clinical relevance. OBJECTIVE: The aim was to contribute to elucidate the complex hypothalamic architecture for research and provide an anatomical basis for clinical brain operation. METHODS: In this research, high-resolution and true-color sectioned images from Visible Korean were employed for hypothalamic nuclei and neighboring structures surface modeling, and a high-resolution three-dimensional atlas of the hypothalamus was created. RESULTS: Surface models of 26 structures including the hypothalamic nuclei and its neighboring structures were produced, which contained 5 anterior hypothalamic areas’ nuclei, 4 intermediate hypothalamic areas’ nuclei, 3 lateral hypothalamic areas’ nuclei, and 2 posterior hypothalamic areas’ nuclei, as well as 12 hypothalamic neighboring structures. CONCLUSIONS: The study evaluated the topographical anatomy of the hypothalamic nuclei and its neighboring structures based on true-color and highresolution sectioned images of Visible Korean.

A monkey phantom is of significant value for electromagnetic radiation (EMR) dosimetry simulations. Furthermore, phantoms in various postures are needed because living beings are exposed to EMR in various postures during their daily routine. In this study, we attempted to produce monkey phantoms based on three daily postures of a rhesus monkey. From our Visible Monkey project, we selected surface models with 177 monkey structures. In the surface models, 52 virtual joints were created to allow for changes from the anatomical position to quadrupedal and sitting positions using commercial software. The surface models of the three positions were converted into monkey voxel phantoms. These phantoms were arranged in three positions, and the number of voxels and mass of each structure were analyzed. The phantoms in anatomical, quadrupedal, and sitting positions have a total of 5,054,022, 5,174,453, and 4,803,886 voxels, respectively. The mass of 177 structures in three positions were also calculated based on the number of voxels. By comparing the monkey phantom with the phantom of a female human, we confirmed thicker skin, less fat, heavier muscle, and a lighter skeleton in monkeys than those in humans. Through posture-transformed monkey phantoms, more precise EMR simulations could be possible. The ultimate purpose of this study is to determine the effects of EMR on humans. For this purpose, we will create posture-transformed human phantoms in a following study using the techniques employed herein and the human phantoms from our previous study.

The Ecklonia cava Kjellman by-product (ECBP) as a feed additive was evaluated in improvement of productivity and immune enhancement against Salmonella Gallinarum (SG). Lohmann Brown chickens proved SGfree were randomly divided into 3 groups of 8 chickens each. Chickens were fed with the experimental diet treatment: T0, Non treatment-commercial feed; T1, commercial feed with 0.5% ECBP; T2, commercial feed with 0.1% Lactobacillus plantarum. In this study, we evaluated the effect of T1 and T2 groups on the body weight and protective efficacy against SG in chickens. The results demonstrated that treatment of T1 group as a feed additive affected significantly body weight gaining in chickens. In addition, T1 group showed a significant different colonization of SG when compared to T2 and T0 groups. We also studied that serum IgG and interferon-γ levels were significantly different compared with other treatment groups. Therefore, we suggest that ECBP can be used as a good candidate of feed additives in chicken industry.

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