Liver kinase B1 (LKB1) has important roles in governing energy homeostasis by regulating the activity of the energy sensor kinase AMP-activated protein kinase (AMPK). The regulation of LKB1 function, however, is still poorly understood. Here we demonstrate that the orphan nuclear receptor Nur77 binds and sequesters LKB1 in the nucleus, thereby attenuating AMPK activation. This Nur77 function is antagonized by the chemical compound ethyl 2-[2,3,4-trimethoxy-6-(1-octanoyl)phenyl]acetate (TMPA), which interacts with Nur77 with high affinity and at specific sites. TMPA binding of Nur77 results in the release and shuttling of LKB1 to the cytoplasm to phosphorylate AMPKα. Moreover, TMPA effectively reduces blood glucose and alleviates insulin resistance in type II db/db and high-fat diet- and streptozotocin-induced diabetic mice but not in diabetic littermates with the Nur77 gene knocked out. This study attains a mechanistic understanding of the regulation of LKB1-AMPK axis and implicates Nur77 as a new and amenable target for the design and development of therapeutics to treat metabolic diseases.

Many kinds of dampers are invented to reduce the dynamic responses due to earthquake or strong wind. Viscoelastic damper is a kind of commonly used passive control device for vibration mitigation of structures. Its energy dissipation characteristics are affected by environmental temperature and excitation frequency. In order to describe the complex characteristics of viscoelastic dampers changing with temperature and frequency, a new model — the equivalent standard solid model, which is based on the standard linear solid model and temperature-frequency equivalent theory, is proposed. The comparison results between experimental and numerical data of viscoelastic dampers show that the equivalent standard solid model can precisely describe energy dissipation behavior of viscoelastic dampers under different temperatures and frequencies. At the same time, 52 viscoelastic dampers are applied for seismic retrofit design on Xi’an Petrol Hotel, and the finite analysis for structures with and without dampers are carried out under frequent-earthquake and infrequent-earthquake excitations. The dynamic responses of structures with and without dampers are compared. Analytical results show that viscoelastic dampers can reduce the earthquake responses effectively, and they are high-performance energy dissipation devices.

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Soil heavy metal pollution has received increasing attention due to their toxicity to soil microorganisms. We have analyzed the effects of heavy metal pollution on ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in soils in the vicinity of a tailings dam of Baotou region, China. Results showed that AOB were dominated with Nitrosomonas-like clusters, while AOA was dominated by group1.1b (Nitrososphaera cluster). Single Cd and Cr contents, as well as compound heavy metal pollution levels, had a significant negative impact on soil potential nitrification rate and both diversities of AOA and AOB. No clear relationship was found between any single heavy metal and abundance of AOA or AOB. But compound pollution could significantly decrease AOA abundance. The results indicated that heavy metal pollution had an obviously deleterious effect on the abundance, diversity, activity and composition of ammonia oxidizers in natural soils.

In spark ignition engines, knock onset limits the maximum spark advance. An inaccurate identification of this limit penalises the fuel conversion efficiency. Thus knock feature extraction is the key of closed-loop control of ignition in spark ignition engine. This paper reports an investigation of knock detection in spark ignition (SI) engines using CEEMD-Hilbert transform based on the engine cylinder pressure signals and engine cylinder block vibration signals. Complementary Ensemble Empirical Mode Decomposition (CEEMD) was used to decompose the signal and detect knock characteristic. Hilbert transform was used to analyze the frequency information of knock characteristic. The result shows that, for both of cylinder pressure signals and vibration signals, the CEEMD algorithm could extract the knock characteristic, and the Hilbert transform result shows that the energy of knock impact areas has the phenomenon of frequency concentration in both cylinder pressure signal and cylinder block vibration signal. At last, the knock window is then determined, based on which a new knock intensity evaluation factorKis propose, and it can accurately distinguish between heavy knock, light knock, and normal combustion three states.