Objective
Develop a fissure sealant containing chitosan/fluoride microparticles (C/F) with antibacterial, fluoride release and recharge ability.
Materials and Methods
Chitosan/fluoride microparticles were synthesized and added to Bis-GMA as C/F. The experimental group comprised 0%, 2%, 4% C/F, with Clinpro
TM
fissure sealant as control. Antibacterial activity was detected by Alamar Blue assay and colony-forming units (CFU). Biocompatibility was determined by WST-1 and LDH test. Curing depth, flowability, tensile strength and flexural strength were measured according to the ISO standard; microhardness by Vickers hardness test. Fluoride release and recharge were recorded through ionic chromatography. Statistical analysis was performed with an independent t-test, one-way and two-way ANOVA. P values less than 0.05 were considered significant.
Results
2% and 4% C/F showed antibacterial ability with CFU ratios decreasing to 10% and 25% respectively (
P
< 0.01). Nonetheless, 4% C/F was concerned because biocompatibility revealed cytotoxicity compared to medium (
P
< 0.001). 2% C/F had superior mechanical properties to Clinpro
TM
fissure sealant in terms of curing depth (
P
< 0.001), microhardness and tensile strength (
P
< 0.01). It had good fluoride release and recharge ability (
P
= 0.67).
Conclusions
2% C/F could be an antibacterial sealant with good mechanical strength, fluoride release and recharge ability.
Introduction:This study investigates the relationship between cellularity and capsular characteristics of pleomorphic adenoma and its influence on operative strategies. Material and methods: The capsular characteristics and clinical data of patients with pleomorphic adenomas were reviewed according to Seifert's definition: (1) classic type with balanced amount of cells and stroma, (2) myxoid type with abundant ground substance, interspersed spindle cells, and (3) cellular type with predominance of ductal trabecular structures and little stroma. The immunoreactivity of cellular proliferation (Ki-67) was semi-quantitatively measured using immunohistochemistry. Variables were analyzed using Fisher's test and one-way ANOVA, with (p < 0.05) considered statistically significant. Results: The duration of presence was associated with cellularity (p = 0.01). In terms of capsular characteristics, satellite nodules and positive resection margins were not related to cellularity, except for incomplete capsules (p = 0.03). There was no difference in the staining scores of . Conclusion: Lower cellularity reflects higher probability of an incomplete capsule, requiring more consideration for operative strategies to prevent recurrence.
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