Summary:been strengthened by several observations showing that growth factors (such as G-CSF, GM-CSF, IL-3 and SCF) modulate the expression or function of several cytoadhesive Transplantation of growth factor-mobilized peripheral blood progenitor cells (PBPC) is widely used in the molecules on the surface of hematopoietic progenitor cells. 8,9 In addition, cytokines produce profound morphotreatment of several neoplastic diseases. While in PBPC harvests the presence of several accessory immune and logical and immunohistochemical changes in marrow stroma and in the contiguous extracellular matrix. 10,11 tumor cells has been documented, that of stromal cells has not been reported. In the present study, we investiThese results suggest that marrow stroma should not be impervious to the effect of growth factors during PBPC gated for the presence of stromal cells in growth factormobilized PBPC harvests from breast cancer patients.mobilization procedures and raise the question of whether stromal cells are released from the marrow and conseLow-density cells from PBCP harvests in culture gave rise to an adherent layer containing fibroblast-like and quently are present in cytokine-mobilized PBPC harvests.In the present study, we monitored the presence of stromal large flat round cells. These cells express positive immunofluorescence staining for collagen I, collagen III, fibcells in growth factor-mobilized PBPC harvests from breast cancer patients. For this purpose, we used a two-step proronectin, VCAM-1 (CD106), ICAM-1 (CD54) and mesenchymal antigens recognized by monoclonal cedure which includes: (1)

Though vascular smooth muscle cell (VSMC) proliferation underlies all cardiovascular hyperplastic disorders, our understanding of the molecular mechanisms responsible for this cellular process is still incomplete. Here we report that SRSF1 (serine/arginine-rich splicing factor 1), an essential splicing factor, promotes VSMC proliferation and injury-induced neointima formation. Vascular injury in vivo and proliferative stimuli in vitro stimulate SRSF1 expression. Mice lacking SRSF1 specifically in SMCs develop less intimal thickening after wire injury. Expression of SRSF1 in rat arteries enhances neointima formation. SRSF1 overexpression increases, while SRSF1 knockdown suppresses the proliferation and migration of cultured human aortic and coronary arterial SMCs. Mechanistically, SRSF1 favours the induction of a truncated p53 isoform, Δ133p53, which has an equal proliferative effect and in turn transcriptionally activates Krüppel-like factor 5 (KLF5) via the Δ133p53-EGR1 complex, resulting in an accelerated cell-cycle progression and increased VSMC proliferation. Our study provides a potential therapeutic target for vascular hyperplastic disease.

Herein we developed a new “smart” Gd-based MR contrast agent (i.e., 1) which is susceptive to furin, a protease overexpressed in tumor. Under the action of furin, 1 condenses to form dimers (1-Ds) and the latter self-assemble into gadolinium nanparticles (Gd-NPs). Relaxivity of 1-D is more than 2 folds of those of 1 and magnevist at 1.5 T, and 1.4 folds of that of 1 at 3 T. Intracellular condensation of 1 in furin-overexpressed MDA-MB-468 cells was proven with direct two-photon laser microscopy (TPLM) fluorescence imaging of the cells incubated with the europium analog of 1 (i.e., 2). Intracellular Gd-NPs of 1 were uncovered and characterized for the first time. MRI of MDA-MB-468 tumors showed that 1 has enhanced MR contrast within the tumors than that of its scrambled control 1-Scr.

In recent years, 3D point clouds have enjoyed a great popularity for representing both static and dynamic 3D objects. When compared to 3D meshes, they offer the advantage of providing a simpler, denser and more close-to-reality representation. However, point clouds always carry a huge amount of data. For a typical example of a point cloud with 0.7 million points per 3D frame at 30 fps, the point cloud raw video needs a bandwidth around 500MB/s. Thus, efficient compression methods are mandatory for ensuring the storage/transmission of such data, which include both geometry and attribute information. In the last years, the issue of 3D point cloud compression (3D-PCC) has emerged as a new field of research. In addition, an ISO/MPEG standardization process on 3D-PCC is currently ongoing. In this paper, a comprehensive overview of the 3D-PCC state-of-the-art methods is proposed. Different families of approaches are identified, described in details and summarized, including 1D traversal compression, 2D-oriented techniques, which take leverage of existing 2D image/video compression technologies and finally purely 3D approaches, based on a direct analysis of the 3D data. CCS CONCEPTS • Computing methodologies → Point-based models; • Information systems → Data compression; • General and reference → Surveys and overviews.