Detection of stromal cells in peripheral blood progenitor cell collections from breast cancer patients

Abstract

Summary:been strengthened by several observations showing that growth factors (such as G-CSF, GM-CSF, IL-3 and SCF) modulate the expression or function of several cytoadhesive Transplantation of growth factor-mobilized peripheral blood progenitor cells (PBPC) is widely used in the molecules on the surface of hematopoietic progenitor cells. 8,9 In addition, cytokines produce profound morphotreatment of several neoplastic diseases. While in PBPC harvests the presence of several accessory immune and logical and immunohistochemical changes in marrow stroma and in the contiguous extracellular matrix. 10,11 tumor cells has been documented, that of stromal cells has not been reported. In the present study, we investiThese results suggest that marrow stroma should not be impervious to the effect of growth factors during PBPC gated for the presence of stromal cells in growth factormobilized PBPC harvests from breast cancer patients.mobilization procedures and raise the question of whether stromal cells are released from the marrow and conseLow-density cells from PBCP harvests in culture gave rise to an adherent layer containing fibroblast-like and quently are present in cytokine-mobilized PBPC harvests.In the present study, we monitored the presence of stromal large flat round cells. These cells express positive immunofluorescence staining for collagen I, collagen III, fibcells in growth factor-mobilized PBPC harvests from breast cancer patients. For this purpose, we used a two-step proronectin, VCAM-1 (CD106), ICAM-1 (CD54) and mesenchymal antigens recognized by monoclonal cedure which includes: (1)

Human peripheral blood derived mesenchymal stem cells demonstrate similar characteristics and chondrogenic differentiation potential to bone marrow derived mesenchymal stem cells

“…In few studies, it has been demonstrated that in a minority of adult marrow cells, CD34 are frequently being expressed. 20 In addition, it has also been described that in 5% of CD34 positive cells, Stro-1 can be identified using monoclonal antibody, which is specific to MSCs. 21 Therefore it is sensible to assume that although this study shows that the cells in culture to be almost lacking of CD34 positive cells, there is still a possibility that non-MSCs have been cultured together in a MSC predominant cell population.…”

Adult Stem Cell Mobilization Enhances Intramembranous Bone Regeneration: A Pilot Study

“…These endogenous progenitor cells can be mobilized to the PB [1,11,12,15,17,22,36,48,52]. Circulating MSCs, and perhaps other adult stem cells, home to sites of skeletal injury [14,23,29,35,45,54], and increasing the number of adult stem cells at sites of injury aids tissue regeneration [4,8,16,40,43,56] either directly or through trophic effects [5,8,17,53].…”

The hematopoietic stroma

“…Differently from hematopoietic cells, the existence of stromal precursors outside the bone marrow in adults is highly controversial, so that while some studies have detected their presence in peripheral blood (53)(54)(55), others have reported negative results (56,57). The existence of stromal precursors in cord blood under normal conditions is the subject of intense discussion (58)(59)(60)(61)(62).…”