HighlightsCoffee was roasted and five typical roasted defects were replicated.Light roast defect had increased indole.Scorched roast defect had increased 4-ethyl-2-methoxyphenol.Dark and baked roast defect had increased phenol and maltol respectively.Underdeveloped roast defect had increased 2,5-dimethylfuran.

The demand for high quality and specialty coffee is increasing worldwide. In order to meet these demands, a more uniform and standardized quality assessment of coffee is essential. The aim of this study was to make a sensory scientific and chemical characterization of common roasting defects in coffee, and to investigate their potential relevance for consumers' acceptance of coffee. To this end, six time-temperature roasting profiles based on a single origin Arabica bean were developed: one 'normal', representing a reference coffee free of defects, and five common roast defects ('dark', 'light', 'scorched', 'baked' and 'underdeveloped'. The coffee samples obtained from these beans were evaluated by means of 1) aroma analysis by Gas Chromatography-Mass Spectrometry (GC-MS), 2) sensory descriptive analysis (DA) by trained assessors, and 3) hedonic and sensory evaluation by consumers using a Check-All-That-Apply (CATA) questionnaire. Multivariate analyses of aroma, DA, and CATA data produced similar sample spaces, showing a clear opposition of the light roast to the dark and scorched roasts), with the normal roast having average values of key aroma compounds. The DA data confirmed this indications and showed the normal roast to have a balanced sensory profile compared to the other defects. Importantly, the normal roast was also significantly preferred in the consumer

HighlightsDifferent levels of acetic acid were used to pre-treat Robusta green coffee beans.Acetic acid pre-treated Robusta had a more similar aroma profile to Arabica.The optimum level of acetic acid treatment was 2%.The maximum level of Robusta coffee added in a blend increased from 20% to 80%.

Highlights:1. Varying levels of sugars were used to pre-treat Robusta green beans.2. Treatment increased the similarity of Robusta to Arabica.3. The optimum level of sugar treatment was Robusta soaked in 15F solution.4. For coffee aroma the blending ratio can be increased from 20% to 80% Robusta.5. The aroma of treated Robusta coffee was more stable than Arabica.

which is critical for its eating quality and understanding the biochemistry and genetics of aroma is important for molecular breeding of millets rich in aroma. In this study, the volatile aroma compounds of the elite millet variety Jingu 21 were investigated at different cooking times, pH, processing methods, and compared with 3 other varieties. An in-vial cooking method was developed which combined solid phase micro-extraction and gas chromatography-mass spectrometry for the detection and identification of volatile compounds. The main findings were: a) Twelve aroma compounds were identified during cooking, which were hexanal, heptanal, octanal, (E)-2-heptenal, nonanal, trans-2-octenal, trans-2-nonenal, 2,4-nonadienal, (E,E)-2,4-decadienal, 1-octen-3-ol, 2-pentylfuran and 6-methyl-5hepten-2-one. b) Longer cooking times produced higher concentrations of aroma compounds. c) Variations in cooking pH (from 6 to 8) had no obvious impact on the aroma of the millet porridge. d) More volatile compounds were released from millet flour compared to millet grain. e) There were significant differences among varieties and Jingu 21 millet showed the highest abundance of most aroma compounds, explaining partly why it is strongly favored by consumers for decades.

Current saliva testing methods rely on cutting edge yet expensive techniques for the detection and analysis of genetic material, proteins and biomarkers for clinical use. However, these techniques are limited in scope and often cannot be used with complex food materials. We propose an efficient ex-vivo tool for evaluating biologically relevant interactions between food components and human saliva using sedimentation velocity analytical ultracentrifugation (SV-AUC). We evaluated macromolecular content from “unstimulated” (US) and “stimulated” (SS) samples pooled from 5 healthy volunteers. Over 90% of total saliva protein consisted of α-amylase and mucin, and up to 10% was secretory immunoglobulin A (SIgA). It was shown that α-amylase concentration increased upon parafilm stimulation, which lead to a decrease in the viscosity of saliva. Then, we used a simple food system (green tea) to evaluate changes in the salivary protein content caused by green tea polyphenols. It was found that aroma release from green tea is highly influenced by interactions between α-amylase and polyphenol epigallocatechin 3-gallate (EGCG). This interaction was found to increase the viscosity of the salivary bulk, suggested to contribute to astringency, and increased the concentrations of β-ionone, benzaldehyde and isovaleraldehyde (P < 0.01), suggested to play a significant role in the characteristic flavour of green tea.

The aroma stability of fresh coffee brew was investigated during storage over 60 minutes, there was a substantial reduction in available 2-furfurylthiol (2-FFT) (84%), methanethiol (72%), 3-methyl-1H-pyrole (68%) and an increase of 2-pentylfuran (65%). It is proposed that 2-FFT was reduced through reversible chemical binding and irreversible losses. Bound 2-FFT was released after cysteine addition, thereby demonstrating that a reversible binding reaction was the dominant mechanism of 2-FFT loss in natural coffee brew. The reduction in available 2-FFT was investigated at different pH and temperatures. At high pH, the reversible binding of 2-FFT was shown to protect 2-FFT from irreversible losses, while irreversible losses led to the reduction of total 2-FFT at low pH. A model reaction system was developed and a potential conjugate, hydroxyhydroquinone, was reacted with 2-FFT. Hydroxyhydroquinone also showed 2-FFT was released after cysteine addition at high pH.

Coffee beans from the same origin were roasted using six time-temperature profiles, in order to identify volatile aroma compounds associated with five common roast coffee defects (light, scorched, dark, baked and underdeveloped). Thirty-seven volatile aroma compounds were selected on the basis that they had previously been identified as potent odorants of coffee and were also identified in all coffee brew preparations; the relative abundance of these aroma compounds was then evaluated using gas chromatography mass spectrometry (GC-MS) with headspace solid phase micro extraction. Some of the 37 key aroma compounds were significantly changed in each coffee roast defect and changes in one marker compound was chosen for each defect type, that is, indole for light defect, 4-ethyl-2-methoxyphenol for scorched defect, phenol for dark defect, maltol for baked defect and 2,5-dimethylfuran for underdeveloped defect. The association of specific changes in aroma profiles for different roast defects has not been shown previously and could be incorporated into screening tools to enable the coffee industry quickly identify if roast defects occur during production.