We propose to exploit multivalent binding of solid-binding
peptides
(SBPs) for the physical attachment of antifouling polypeptide brushes
on solid surfaces. Using a silica-binding peptide as a model SBP,
we find that both tandem-repeated SBPs and SBPs repeated in branched
architectures implemented via a multimerization domain work very well
to improve the binding strength of polypeptide brushes, as compared
to earlier designs with a single SBP. At the same time, for many of
the designed sequences, either the solubility or the yield of recombinant
production is low. For a single design, with the domain structure
B
-
M
-
E
, both solubility and yield of recombinant production
were high. In this design,
B
is a silica-binding
peptide,
M
is a highly thermostable,
de novo-designed trimerization domain, and
E
is a hydrophilic elastin-like polypeptide. We show that the
B
-
M
-
E
triblock polypeptide rapidly assembles into highly
stable polypeptide brushes on silica surfaces, with excellent antifouling
properties against high concentrations of serum albumin. Given that
SBPs attaching to a wide range of materials have been identified,
the
B
-
M
-
E
triblock design provides a template
for the development of polypeptides for coating many other materials
such as metals or plastics.
Catalytic and electrocatalytic applications of supported metal nanoparticles are hindered due to an aggregation of metal nanoparticles and catalytic leaching under harsh operations. Hence, stable and leaching free catalysts with high surface area are extremely desirable but also challenging. Here we report a gold nanoparticles-hosted mesoporous nitrogen doped carbon matrix, which is prepared using bovine serum albumin (BSA) through calcination. BSA plays three roles in this process as a reducing agent, capping agent and carbon precursor, hence the protocol exhibits economic and sustainable. Gold nanoparticles at N-doped BSA carbon (AuNPs@NBSAC)-modified three-electrode strip-based flexible sensor system has been developed, which displayed effective, sensitive and selective for simultaneous detection of uric acid (UA) and dopamine (DA). The AuNPs@NBSAC-modified sensor showed an excellent response toward DA with a linear response throughout the concentration range from 1 to 50 μM and a detection limit of 0.05 μM. It also exhibited an excellent response toward UA, with a wide detection range from 5 to 200 μM as well as a detection limit of 0.1 μM. The findings suggest that the AuNPs@NBSAC nanohybrid reveals promising applications and can be considered as potential electrode materials for development of electrochemical biosensors.
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