SUMMARYChronic grafl-vcnwA-host disease (GVHD) was induced in female (C57 B10S/DBA/2)F| hybrid mice with two successive injections of lymphoid cells from parental DBA/2 strain. Serial bleedings of 27 GVHD mice were screened with a panel of antigens including the five histones HI, H2A, H2B, H3 and H4, 15 histonc peptides. core particles. dsDNA. heat-shoek proteins hsp70 and ubiquitin, a branched peptide of ubiquitinaled H2A (U-H2A), poly(ADP-ribose) and SSB/l,a protein. The predominant IgG response to histone peptides was directed against regions 204-218 of HI, 1-25 of H2B and 1 29 of H4. GVHD mice also produced IgG antibodies lo dsDNA and chromatin core particles as reported previously. IgG antibodies reacting with dsDNA appeared before antibodies to core particles and histones. Raised levels ofantibodies to U'H2A. but not to monomeric ubiquitin. were also found. While the level ofantibodies to dsDNA. histones and core particles decreased significantly before ihe appearance of proteinuria, suggesting their involvement in glomerular injury, the longitudinal pattern of anti-U-H2A peptide response was apparently not linked to the manifestation of lupus nephritis in GVHD mice.
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