The transition from outcrossing to self-fertilization is one of the most common evolutionary trends in plants. Reproductive assurance, where self-fertilization ensures seed production when pollinators and/or potential mates are scarce, is the most long-standing and most widely accepted explanation for the evolution of selfing, but there have been few experimental tests of this hypothesis. Moreover, many apparently adaptive floral mechanisms that ensure the autonomous production of selfed seed might use ovules that would have otherwise been outcrossed. This seed discounting is costly if selfed offspring are less viable than their outcrossed counterparts, as often happens. The fertility benefit of reproductive assurance has never been examined in the light of seed discounting. Here we combine experimental measures of reproductive assurance with marker-gene estimates of self-fertilization, seed discounting and inbreeding depression to show that, during 2 years in 10 Ontario populations of Aquilegia canadensis (Ranunculaceae), reproductive assurance through self-fertilization increases seed production, but this benefit is greatly outweighed by severe seed discounting and inbreeding depression.
Biogeographic models predict that geographically peripheral populations should be smaller, more sparsely distributed, and have a lower per-capita reproductive rate than populations near the center of a species' range. Plants in peripheral populations may, therefore, receive less pollinator visitation and outcross pollination, which may select for self-fertilization to provide reproductive assurance. We tested these predictions by comparing population size, plant density, seed production, floral traits, and mating system parameters between 10 populations of Aquilegia canadensis near the northern margin of the range with 10 near the range center. Contrary to predictions, peripheral populations were not smaller, less dense, nor less productive than central populations. Nevertheless, we detected substantial regional differences in key floral traits. Plants in central populations produced larger flowers with 68% greater herkogamy and had 30% more flowers open simultaneously than plants in northern populations. However, there was no regional difference in the mating system. In northern populations, 73% (range = 60-88%) of seeds were self-fertilized compared to 76% (51-100%) in central populations. In both regions, adult inbreeding coefficients were near zero, indicating very strong inbreeding depression despite high selfing. Marked geographic variation in key floral traits does not reflect evolutionary differentiation in the mating system.
The mating system of flowering plant populations evolves through selection on genetically based phenotypic variation in floral traits. The physical separation of anthers and stigmas within flowers (herkogamy) is expected to be an important target of selection to limit self-fertilization. We investigated the pattern of phenotypic and genetic variation in herkogamy and its effect of selffertilization in a broad sample of natural populations of Aquilegia canadensis, a species that is highly selfing despite strong inbreeding depression. Within natural populations, plants exhibit substantial phenotypic variation in herkogamy caused primarily by variation in pistil length rather than stamen length. Compared to other floral traits, herkogamy is much more variable and a greater proportion of variation is distributed among rather than within individuals. We tested for a genetic component of this marked phenotypic variation by growing naturally pollinated seed families from five populations in a common greenhouse environment. For three populations, we detected a significant variation in herkogamy among families, and a positive regression between parental herkogamy measured in the field and progeny herkogamy in the greenhouse, suggesting that there is often genetic variation in herkogamy within natural populations. We estimated levels of self-fertilization for groups of flowers that differed in herkogamy and show that, as expected, herkogamy was associated with reduced selfing in 13 of 19 populations. In six of these populations, we performed floral emasculations to show that this decrease in selfing is due to decreased autogamy (within-flower selfing), the mode of selfing that herkogamy should most directly influence. Taken together, these results suggest that increased herkogamy should be selected to reduce the production of low-quality selfed seed. The combination of high selfing and substantial genetic variation for herkogamy in A. canadensis is enigmatic, and reconciling this observation will require a more integrated analysis of how herkogamy influences not only self-fertilization, but also patterns of outcross pollen import and export.KEY WORDS: Aquilegia canadensis, autogamy, heritability, herkogamy, inbreeding depression, outcrossing, self-fertilization.Evolution of the tremendous diversity of mating systems exhibited by flowering plants involves natural selection acting on phenotypic variation in plant traits such as floral morphology, devel-
Summary Intralocus sexual conflict is a form of conflict that does not involve direct interactions between males and females. It arises when selection on a shared trait with a common genetic basis differs between the sexes. Environmental factors, such as resource availability, may influence the expression and evolutionary outcome of such conflict. We quantified the genetic variance‐covariance matrix, G, for both sexes of Silene latifolia for floral and leaf traits, as well as the between‐sex matrix, B. We also quantified selection on the sexes via survival for 2 yr in four natural populations that varied in water availability. Environment‐dependent intralocus sexual conflict exists for specific leaf area, a trait that is genetically correlated between the sexes. Males experienced significant negative selection, but only in populations with relatively limited water availability. Females experienced weakly positive or significant stabilizing selection on the same trait. Specific leaf area is genetically correlated with flower size and number, which are sexually dimorphic in this species. The extent of intralocus sexual conflict varied with the environment. Resolution of such conflict is likely to be confounded, given that specific leaf area is highly genetically integrated with other traits that are also divergent between the sexes.
The evolution of sexual dimorphism will depend on how sexual, fecundity and viability selection act within each sex, with the different forms of selection potentially operating in opposing directions. We examined selection in the dioecious plant Silene Together with previous results they suggest that the outcome of the different forms of selection will be environmentally dependent, and therefore help to explain variation among populations in sexually dimorphic traits.
Genetic correlations between the sexes can constrain the evolution of sexual dimorphism and be difficult to alter, because traits common to both sexes share the same genetic underpinnings. We tested whether artificial correlational selection favoring specific combinations of male and female traits within families could change the strength of a very high between-sex genetic correlation for flower size in the dioecious plant Silene latifolia. This novel selection dramatically reduced the correlation in two of three selection lines in fewer than five generations. Subsequent selection only on females in a line characterized by a lower between-sex genetic correlation led to a significantly lower correlated response in males, confirming the potential evolutionary impact of the reduced correlation. Although between-sex genetic correlations can potentially constrain the evolution of sexual dimorphism, our findings reveal that these constraints come not from a simple conflict between an inflexible genetic architecture and a pattern of selection working in opposition to it, but rather a complex relationship between a changeable correlation and a form of selection that promotes it. In other words, the form of selection on males and females that leads to sexual dimorphism may also promote the genetic phenomenon that limits sexual dimorphism. K E Y W O R D S :Between-sex genetic correlation, dioecious, response to selection, sexual dimorphism.
Abstract. Inbreeding is a major component of the mating system in populations of many plants and animals, particularly hermaphroditic species. In flowering plants, inbreeding can occur through self-pollination within flowers (autogamy), self-pollination between flowers on the same plant (geitonogamy), or cross-pollination between closely related individuals (biparental inbreeding). We performed a floral emasculation experiment in 10 populations of Aquilegia canadensis (Ranunculaceae) and used allozyme markers to estimate the relative contribution of each mode of inbreeding to the mating system. We also examined how these modes of inbreeding were influenced by aspects of population structure and floral morphology and display predicted to affect the mating system. All populations engaged in substantial inbreeding. On average, only 25% of seed was produced by outcrossing (range among populations ϭ 9-37%), which correlated positively with both population size (r ϭ ϩ0.61) and density (r ϭ ϩ0.64). Inbreeding occurred through autogamy and biparental inbreeding, and the relative contribution of each was highly variable among populations. Estimates of geitonogamy were not significantly greater than zero in any population. We detected substantial biparental inbreeding (mean ϭ 14% of seeds, range ϭ 4-24%) by estimating apparent selfing in emasculated plants with no opportunity for true selfing. This mode of inbreeding correlated negatively with population size (r ϭ Ϫ0.87) and positively with canopy cover (r ϭ ϩ0.90), suggesting that population characteristics that increase outcross pollen transfer reduce biparental inbreeding. Autogamy was the largest component of the mating system in all populations (mean ϭ 58%, range ϭ 37-84%) and, as expected, was lowest in populations with the most herkogamous flowers (r ϭ Ϫ0.59). Although autogamy provides reproductive assurance in natural populations of A. canadensis, it discounts ovules from making superior outcrossed seed. Hence, high autogamy in these populations seems disadvantageous, and therefore it is difficult to explain the extensive variation in herkogamy observed both among and especially within populations.Key words. Aquilegia canadensis, autogamy, biparental inbreeding, geitonogamy, herkogamy, inbreeding, matingsystem evolution, self-fertilization. Inbreeding is a major component of the mating system in many plants and animals, and can profoundly influence key ecological and evolutionary processes (Thornhill 1993). Inbreeding via self-fertilization is particularly prevalent in plants because most species are hermaphroditic, and many are self compatible . In general, an allele that causes self-fertilization will often be selected because it increases its own transmission by avoiding the genetic cost of outcrossing (Fisher 1941). This advantage is balanced by inbreeding depression: the reduced survival and/ or reproductive success of selfed compared to outcrossed progeny (Charlesworth and Charlesworth 1987). The fitness consequences of selfing also depend on the details of...
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