The rejoining of DNA double strand breaks (dsb) induced by 60Co gamma-rays, 241Am alpha-particles or bleomycin was measured by neutral filter elution. In agreement with their colony-forming ability, ataxia-telangiectasia cells (AT2BE) and normal fibroblasts exhibited similar dsb rejoining capacity following alpha-irradiation, but showed marked differences in the rejoining kinetics of dsb induced by gamma-rays or bleomycin.
Purified, dry DNA and dry nucleoprotein were irradiated with 60Co-γ-rays under a nitrogen or oxygen atmosphere. The DNA was isolated from the irradiated nucleoprotein. In the DNA the following radiation induced changes were investigated: Double strand breaks, single strand breaks and crosslinks between the DNA molecules. An oxygen effect (OER > 1) was found for all of these events except for crosslinks in irradiated DNA. In the nucleoprotein, the oxygen effect is more marked than in pure DNA.
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