At the end of twenty-four days, two plants from each treatment were supplied with 0.31 ~tci/ml of radioactive iron (5~Fe) (with the same P levels used to grow them) and then allowed to absorb the 55Fe for 3 hours after which they were harvested, separated into roots and shoots.The two components were wet-ashed separately, and assayed for 5SFe. The rest of the plants in Hoagland solution were also harvested, separately into roots and shoots. The components parts were also wet-ashed separately and assayed for iron (Fe), manganese (Mn), zinc (Zn), copper (Cu) and phosphorous (P).High P concentration in the growth medium inhibited ~ 5 Fe absorption and translocation in both millet and sorghum but millet was more tolerant of P-induced Fe deficiency than sorghum.Millet and sorghum grew best at 1.0 and 0.1 mM P, respectively. Fe concentrations in plant declined as P levels increased; while Mn increased in both millet and sorghum with increasing P levels. Millet, however, maintained higher concentrations of Fe and Mn.The distribution patter of 5SFe, regardless of P levels could be summarized as follows: Roots > Vascular tissue > leaf-sheaths > leaf blade. Less 55Fe was absorbed and translocated to shoots at 2.5 mM P, in millet and sorghum.
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