The synthesis of metal nanoparticles has become a priority for the advancement of nanotechnology. In attempts to create these nanoparticles, several different methods: chemistry, physics, and biology, have all been...
Many research groups have attained slow, persistent, continuous release of silver ions through careful experimental design using existing methods. Such methods effectively kill planktonic bacteria and therefore prevent surface adhesion of pathogens. However, the resultant modified coatings cannot provide long-term antibacterial efficacy due to sustained anti-microbial release. In this study, the anti-infection activity of AgNP immobilized biomaterials was evaluated, facilitated by argon plasma grafting technology and activated by bacterial colonization. The modified materials generated in this study showed excellent specificity and were active against both Gram-positive and Gram-negative biofilm forming bacteria, including methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli. The anti-infection biomaterials developed in this study demonstrate several attractive advantages in comparison to traditional anti-bacterial surfaces loaded with antibiotics or other types of antibacterial agents and include (1) broad spectrum of activity against antibiotic resistant bacteria, (2) the unlikelihood of bacterial resistance, (3) specificity, (4) biocompatibility, and (5) stability.
Formation of bacterial biofilms at solid-liquid interfaces creates numerous problems in both industrial and biomedical sciences. In this study, the susceptibility of Staphylococcus aureus biofilms to discharge gas generated from plasma was tested. It was found that despite distinct chemical/physical properties, discharge gases from oxygen, nitrogen, and argon demonstrated very potent and almost the same anti-biofilm activity. The bacterial cells in S. aureus biofilms were killed (>99.9%) by discharge gas within minutes of exposure. Under optimal experimental conditions, no bacteria and biofilm re-growth from discharge gas treated biofilms was found. Further studies revealed that the anti-biofilm activity of the discharge gas occurred by two distinct mechanisms: 1) killing bacteria in biofilms by causing severe cell membrane damage, and 2) damaging the extracellular polymeric matrix in the architecture of the biofilm to release biofilm from the surface of the solid substratum . Information gathered from this study provides an insight into the anti-biofilm mechanisms of plasma and confirms the applications of discharge gas in the treatment of biofilms and biofilm related bacterial infections.
The antibacterial and anti-biofilm activities of propolis have been intensively reported. However, the application of this folk remedy as a means to prevent biomedical implant contamination has yet to be completely evaluated. In response to the significant resistant and infectious attributes of biofilms, biomaterials engineered to possess specific chemical and physical properties were immobilized with metal free Russian propolis ethanol extracts (MFRPEE), a known antibacterial agent. The results obtained from this study begin to examine the application of MFRPEE as a novel alternative method for the prevention of medical and biomedical implant infections. When constructed under specific experimental conditions, immobilized biomaterials showed excellent stability when subjected to simulated body fluid and fetal bovine serum. The ability of immobilized biomaterials to specifically target pathogens (both Gram-positive and Gram-negative biofilm forming bacteria), while promoting tissue cell growth, renders these biomaterials as potential candidates for clinical applications.
Indwelling device infections now represents life-threatening circumstances as a result of the biofilms’ tolerance to antibiotic treatments. Current antibiotic impregnation approaches through sustained antibiotic release have some unsolved problems which include short life-span, narrowed antibacterial spectrum, ineffectiveness towards resistant mutants, and the potential to hasten the antibiotic resistance process. In this study, bacteria responsive anti-biofilm surfaces were developed using bioactive peptides with proved activity to antibiotic resistant bacteria and biofilms. Resulting surfaces were stable under physiological conditions and in the presence of high concentrations of salts (0.5 M NaCl) and biomacromolcules (1.0% DNA and 2.0% alginate), and thus showed good biocompatibility to various tissue cells. However, lytic peptide immobilized surfaces could sense bacteria adhesion and kill attached bacteria effectively and specifically, so biofilms were unable to develop on the lytic peptide immobilized surfaces. Bacteria responsive catheters remained biofilm free for up to a week. Therefore, the bacteria responsive antibacterial surfaces developed in this study represent new opportunities for indwelling device infections.
Most strains of bacteria and subsequently biofilms, have evolved and have altered their chemical composition in an attempt to protect themselves from antibiotics. The resistant nature of bacteria stems from the chemical rather than the physical means of inactivation of antibiotics. The results uncovered in this work demonstrate the potential application of Russian propolis ethanol extracts as a very efficient and effective method for bacterial and biofilm inactivation.
The
discovery of new and versatile strategies for the immobilization
of molecular water-oxidation catalysts (WOCs) is crucial for developing
clean energy conversion devices [e.g., (photo)electrocatalytic cells
for water splitting]. The traditional approach for surface attachment
to transparent conductive oxides [e.g., fluorine doped tin oxide (FTO)]
is via synthetic modification of the ligand architecture to incorporate
functional groups such as carboxylic acids (−COOH) or phosphonates
(−PO3H2) prior to immobilization. However,
challenges arising from desorption and the cumbersome derivatizations
steps have limited the scope and applications of surface-bound WOCs.
Herein, we report the successful immobilization of underivatized Ru(II)-based
WOCs (Ru–Cat1 = [Ru(tpy) (bpy) (H2O)]2+ (tpy = 2,2′:6′2″–terpyridine
and bpy = 2,2′-bipyridine) and Ru–Cat2 =
[Ru(Mebimpy) (bpy) (H2O)]2+ (Mebimpy = 2,6-bis(1-methylbenzimidazol-2-yl)
pyridine)) and the Ru(II) polypyridyl chromophore Ru–C3 = [Ru(bpy)3]2+ onto a FTO plasma-grafted poly(acrylic
acid) surface (PAA|FTO). Various characterization techniques such
as attenuated total reflectance Fourier transform infrared spectroscopy,
scanning electron microscopy, atomic force microscopy, and cyclic
voltammetry measurements provide evidence for the plasma-induced grafted
PAA|FTO film and immobilization. Surface stability and electrocatalytic
properties of these new hybrid composite films upon cycling were investigated
at different pH values. Immobilized Ru–Cat1 and Ru–Cat2 onto PAA|FTO displayed pH-dependent (RuIII/RuII) couples and onset potentials indicative
of PCET (proton-coupled electron transfer) reactions. Based on cyclic
voltammetry results and spectroscopic monitoring, the immobilized
WOCs Ru–Cat1 and Ru–Cat2 exhibited
a higher surface stability in neutral aqueous solutions relative to Ru–C3 upon electrochemical oxidation. We attribute
the surface PCET and stability to the presence of a water ligand in
the coordination sphere of immobilized Ru–Cat1 and Ru–Cat2 which can H-bond with negatively
charged carboxylate groups of the cross-linked PAA brushes. Our findings
demonstrate that the plasma-grafted polymeric network onto FTO offers
a versatile platform to directly anchor unmodified homogeneous WOCs
or chromophores for potential applications in solar-to-fuel energy
conversion.
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