Bacteria responsive antibacterial surfaces for indwelling device infections

Abstract

Indwelling device infections now represents life-threatening circumstances as a result of the biofilms’ tolerance to antibiotic treatments. Current antibiotic impregnation approaches through sustained antibiotic release have some unsolved problems which include short life-span, narrowed antibacterial spectrum, ineffectiveness towards resistant mutants, and the potential to hasten the antibiotic resistance process. In this study, bacteria responsive anti-biofilm surfaces were developed using bioactive peptides with proved activity to antibiotic resistant bacteria and biofilms. Resulting surfaces were stable under physiological conditions and in the presence of high concentrations of salts (0.5 M NaCl) and biomacromolcules (1.0% DNA and 2.0% alginate), and thus showed good biocompatibility to various tissue cells. However, lytic peptide immobilized surfaces could sense bacteria adhesion and kill attached bacteria effectively and specifically, so biofilms were unable to develop on the lytic peptide immobilized surfaces. Bacteria responsive catheters remained biofilm free for up to a week. Therefore, the bacteria responsive antibacterial surfaces developed in this study represent new opportunities for indwelling device infections.

The mechanism of action of Russian propolis ethanol extracts against two antibiotic-resistant biofilm-forming bacteria

“…In the MTT assay, biofilms were incubated with MTT at 37°C for 30 min. After washing, the purple formazan formed inside the bacterial cells was dissolved by SDS and then measured using a microplate reader by setting the detecting and reference wavelengths at 570 and 630 nm respectively (Traba and Liang ).…”

Targeting of Nanotherapeutics to Infection Sites for Antimicrobial Therapy

“…Traba et al. developed responsive antibacterial surfaces by coating polyethylene terephthalate (PET) with lytic peptides because they demonstrated good antibacterial properties . A surface coating using this material showed excellent antibacterial and antibiofilm activity compared with an unmodified surface.…”

Smart Drug Release from Medical Devices

“…The surface of medical devices can be easily modified with poly(acrylic acid) (PAA) by use of either plasma polymerization or gamma-ray grafting approaches; PAA-grafted chains act as intermediates for binding cationic drugs through strong ionic interactions (Mendes, 2008;Alvarez-Lorenzo et al, 2010). Remarkably, changes in pH in the typical range of microorganism growth may reverse the affinity and thus trigger the release of the drug, as demonstrated for vancomycin and antimicrobial peptides (Ruiz et al, 2008;Muñoz-Muñoz et al, 2012;García-Vargas et al, 2014;Traba and Liang, 2015). In case of the lytic peptide agents, their direct transfer from the device to the bacteria membrane and the increase in their activity as the pH decreases strongly contributes to the inhibition of biofilm formation (Traba and Liang, 2015).…”

“…Remarkably, changes in pH in the typical range of microorganism growth may reverse the affinity and thus trigger the release of the drug, as demonstrated for vancomycin and antimicrobial peptides (Ruiz et al, 2008;Muñoz-Muñoz et al, 2012;García-Vargas et al, 2014;Traba and Liang, 2015). In case of the lytic peptide agents, their direct transfer from the device to the bacteria membrane and the increase in their activity as the pH decreases strongly contributes to the inhibition of biofilm formation (Traba and Liang, 2015).…”