A procedure for the real-time analysis of laser modes using a phase-only spatial light modulator is outlined. The procedure involves encoding into digital holograms by complex amplitude modulation a set of orthonormal basis functions into which the initial field is decomposed. This approach allows any function to be encoded and refreshed in real time (60 Hz). We implement a decomposition of guided modes propagating in optical fibers and show that we can successfully reconstruct the observed field with very high fidelity.

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We demonstrate a versatile method for the measurement of the orbital angular momentum (OAM) density of an optical field. By performing a modal decomposition with digital holograms, we reconstruct the full optical field from a small set of single-point intensity measurements, from which optical vortices, global OAM and OAM density can be derived. We validate the method on defined OAM-carrying beams yielding fidelities in the OAM density measurement of up to 99%, and subsequently apply the technique to unknown fields from optical fibers.

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Changes in mechanical properties are an essential characteristic of the aging process of human skin. Previous studies attribute these changes predominantly to the altered collagen and elastin organization and density of the extracellular matrix. Here, we show that individual dermal fibroblasts also exhibit a significant increase in stiffness during aging in vivo. With the laser-based optical cell stretcher we examined the viscoelastic biomechanics of dermal fibroblasts isolated from 14 human donors aged 27 to 80. Increasing age was clearly accompanied by a stiffening of the investigated cells. We found that fibroblasts from old donors exhibited an increase in rigidity of ∼60% with respect to cells of the youngest donors. A FACS analysis of the content of the cytoskeletal polymers shows a shift from monomeric G-actin to polymerized, filamentous F-actin, but no significant changes in the vimentin and microtubule content. The rheological analysis of fibroblast-populated collagen gels demonstrates that cell stiffening directly results in altered viscoelastic properties of the collagen matrix. These results identify a new mechanism that may contribute to the age-related impairment of elastic properties in human skin. The altered mechanical behavior might influence cell functions involving the cytoskeleton, such as contractility, motility, and proliferation, which are essential for reorganization of the extracellular matrix.

The functional loss of mitochondria represents an inherent part in modern theories trying to explain the cutaneous aging process. The present study shows significant age-dependent differences in mitochondrial function of keratinocytes isolated from skin biopsies of young and old donors. Our data let us postulate that energy metabolism shifts to a predominantly non-mitochondrial pathway and is therefore functionally anaerobic with advancing age. CoQ10 positively influences the age-affected cellular metabolism and enables to combat signs of aging starting at the cellular level. As a consequence topical application of CoQ10 is beneficial for human skin as it rapidly improves mitochondrial function in skin in vivo.

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