The objective of this research was to obtain and compare constant and variable amplitude fatigue behavior of AZ91E-T6 cast magnesium alloy in both an air and 3.5 percent NaCl aqueous corrosive environment. An additional objective was to determine if commonly used models that describe fatigue behavior and fatigue life are applicable to this material and test environment. Fatigue tests included constant amplitude strain-controlled low cycle fatigue with strain ratio, R, equal to 0, −1 and −2, Region II constant amplitude fatigue crack growth with load ratio, R, equal to 0.05 and 0.5 and variable amplitude fatigue tests using keyhole notched specimens. In all fatigue tests, the corrosion environment was significantly detrimental relative to the air environment. Mean strains influenced fatigue life only if accompanied by significant mean stress. The Morrow and Smith, Watson, and Topper mean stress models provided both accurate and inaccurate fatigue life calculations. Likewise, variable amplitude fatigue life calculations using the local strain approach and based upon the formation ofal mm crack at the keyhole notch were both accurate and fairly inaccurate depending on the specific model used.
Biodegradable magnesium alloys, Mg-0·8wt%Ca and Mg-10wt%Gd, have been coated with gallium-containing electrolyte using plasma electrolytic oxidation (PEO). Thus, the PEO procedure is used as a synthesis tool, to create an appropriate content in the layers. This is a first article on the examination of such layers. One part of the gallium-coated samples was additionally sealed with biodegradable poly(L-lactide-co-caprolactone) (PLLC). Gallium content, morphology and thickness of the coatings were evaluated. Special focus was set in relation to the cytotoxicity of the coating, on the two different magnesium alloys and the additional sealing with PLLC. Cytotoxicity was tested using live/dead staining of MC3T3-E1 fibroblasts and by WST-1 assay, cell differentiation by alkaline phosphatase measurement. It could be shown that the gallium-ion concentration in the PEO layer was higher for Mg-10wt%Gd than that of Mg-0·8wt%Ca, which caused a significant difference in the cytotoxic effects toward MC3T3-E1 cells. Still in all cytotoxic tests, Mg-10wt%Gd showed no to low cytotoxic levels, whereby Mg-0·8wt%Ca showed moderate results. Furthermore, it could be proved that an additional PLLC sealing benefits the cell adhesion and thus promotes the cytocompatibility of the coating.
Coating of plasma chemical oxidized titanium (TiOB®) with gentamicin-tannic acid (TiOB® gta) has proven to be efficient in preventing bacterial colonization of implants. However, in times of increasing antibiotic resistance, the development of alternative antimicrobial functionalization strategies is of major interest. Therefore, the aim of the present study is to evaluate the antibacterial and biocompatible properties of TiOB® functionalized with silver nanoparticles (TiOB® SiOx Ag) and ionic zinc (TiOB® Zn). Antibacterial efficiency was determined by agar diffusion and proliferation test on Staphylocuccus aureus. Cytocompatibility was analyzed by direct cultivation of MC3T3-E1 cells on top of the functionalized surfaces for 2 and 4 d. All functionalized surfaces showed significant bactericidal effects expressed by extended lag phases (TiOB® gta for 5 h, TiOB® SiOx Ag for 8 h, TiOB® Zn for 10 h). While TiOB® gta (positive control) and TiOB® Zn remained bactericidal for 48 h, TiOB® SiOx Ag was active for only 4 h. After direct cultivation for 4 d, viable MC3T3-E1 cells were found on all surfaces tested with the highest biocompatibility recorded for TiOB® SiOx Ag. The present study revealed that functionalization of TiOB® with ionic zinc shows bactericidal properties that are comparable to those of a gentamicin-containing coating.
The surface properties of titanium alloy implants for improved osseointegration in orthopaedic and dental surgery have been modified by many technologies. Hydroxyapatite coatings with a facultative integration of growth factors deposited by plasma spraying showed improved osseointegration. Our approach in order to enhance osseointegration was carried out by a surface modification method of titanium alloy implants called plasma chemical oxidation (PCO). PCO is an electrochemical procedure that converts the nm‐thin natural occurring titanium‐oxide layer on an implant to a 5 µm thick ceramic coating (TiOB‐surface). Bioactive TiOB‐surfaces have a porous microstructure and were loaded with calcium and phosphorous, while bioinert TiOB‐surfaces with less calcium and phosphorous loadings are smooth. A rat tibial model with bilateral placement of titanium alloy implants was employed to analyze the bone response to TiOB‐surfaces in vivo. 64 rats were randomly assigned to four groups of implants: (i) pure titanium alloy (control), ii) titanium alloy, type III anodization, (iii) bioinert TiOB‐surface, and (iv) bioactive TiOB‐surface. Mechanical fixation was evaluated by pull out tests at 3 and 8 weeks.
The bioactive TiOB‐surface showed significantly increased shear strength at 8 weeks compared to all other groups.
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