BackgroundExosomes are extracellular vesicles released by almost all cell types, including cancer cells, into bodily fluids such as saliva, plasma, breast milk, semen, urine, cerebrospinal fluid, amniotic fluid, synovial fluid and sputum. Their key function being intercellular communication with both neighbouring as well as distant cells. Cancer exosomes have been shown to regulate organ-specific metastasis. However, little is known about the functional differences and molecular consequences of normal cells responding to exosomes derived from normal cells compared to those derived from cancer cells.MethodsHere, we characterised and compared the transcriptome profiles of primary human normal oral keratinocytes (HNOK) in response to exosomes isolated from either primary HNOK or head and neck squamous cell carcinoma (HNSCC) cell lines.ResultsIn recipient HNOK cells, we found that regardless of normal or cancer derived, exosomes altered molecular programmes involved in matrix modulation (MMP9), cytoskeletal remodelling (TUBB6, FEZ1, CCT6A), viral/dsRNA-induced interferon (OAS1, IFI6), anti-inflammatory (TSC22D3), deubiquitin (OTUD1), lipid metabolism and membrane trafficking (BBOX1, LRP11, RAB6A). Interestingly, cancer exosomes, but not normal exosomes, modulated expression of matrix remodelling (EFEMP1, DDK3, SPARC), cell cycle (EEF2K), membrane remodelling (LAMP2, SRPX), differentiation (SPRR2E), apoptosis (CTSC), transcription/translation (KLF6, PUS7). We have also identified CEP55 as a potential cancer exosomal marker.ConclusionsIn conclusion, both normal and cancer exosomes modulated unique gene expression pathways in normal recipient cells. Cancer cells may exploit exosomes to confer transcriptome reprogramming that leads to cancer-associated pathologies such as angiogenesis, immune evasion/modulation, cell fate alteration and metastasis. Molecular pathways and biomarkers identified in this study may be clinically exploitable for developing novel liquid-biopsy based diagnostics and immunotherapies.Electronic supplementary materialThe online version of this article (10.1186/s12943-018-0846-5) contains supplementary material, which is available to authorized users.
Introduction: Cholesterol is a fat found in our body, it can be dangerous if cholesterol level is increasing. The way to lower cholesterol levels, such as by consuming high-fiber foods for example red guava. Purpose: To determine the difference in total cholesterol levels in rats after giving red guava juice. Method: The research was conducted using 30 Rattus Norvegicus. Rats were divided into three groups, each group consisted of 10 rats. Control group was given 594 chicken feed, first treatment group was given lard and second treatment group was given lard with red guava juice. Before being given treatment, five rats from each group were examined for their total cholesterol and the remaining five rats were given treatment for 14 days before total cholesterol were checked. Examination of total cholesterol by enzymatic colorimetry CHOD-PAP using blood from orbital vein of rats without fasting. Result: There were significant differences in total cholesterol of rats before and after treatment (P<0.05) in each treatment group. In comparison of three groups that had given treatment, significant difference was found (P=0.005). Significant differences were also found in comparison of two groups, between first treatment group and second treatment group (P = 0.004), insignificant results were found in comparison of control group with first treatment group (P = 0.060), and comparison of control group with second treatment group. (P=0.299). Conclusion: There are differences in total cholesterol of rats, total cholesterol of rats in high-fat diet group with guava juice were lower than the high-fat diet group.
Introduction: Indonesian Ministry of Health Research stated that in 2017 the population of the elderly reached 23.66 million people (9.03%) and also predicted that it will continue to increase. The aging process causes declined physical capacity and mental, which makes the elderly have a higher risk of falls. One of the preventions of falls in the elderly is physical exercise to increase muscle strength and joint mobility. Chair-based Exercise is a physical exercise done by sitting, consisting of aerobic and muscle resistance exercise with Thera Band. Purpose: To analyze the risk of falls in the elderly before and after Chair Based Exercise. Method: This study used literature synthesis. Keywords collected the Articles due to the topic in the research proposal. Result: 13 articles were reviewed in this study. Most articles have similarities in research design, analysis techniques, sample characteristics, total sample, duration of the intervention, and effect of the intervention studied. The differences mainly on the age of the sample and the intervention method. The similarities between the articles and research proposal mostly on sample characteristics and measuring the risk of falls. The difference mostly in a total of the sample, research design, analysis technique, duration and method of the intervention. Conclusion: Only a few articles published Chair Based Exercise as an intervention method. There are similarities in the interventions given in the form of combination training, and it was found that the risk of falls was improved in the elderly. Therefore, research on the effect of Chair Based Exercise on the risk of falls in the elderly needs to be realized by enriching various inputs from literature studies.
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