Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.
The purpose of this study is to explore the impact of the use motivation of Sports Intelligent Wearable Equipment on target orientation and Sports involvement. Taking the users of sports intelligent wearable equipment as the research object, the researchers were asked to send questionnaires and network questionnaires, total of 400 questionnaires were issued, 385 valid questionnaires were collected, the effective recovery rate was 96.25%. Data processing use descriptive statistics, independent sample t test, single factor variance analysis and multiple stepwise regression analysis. The research results are: first, male was mainly using the movement of intelligent wearable equipment: for the most age were 21 to 25 years old; most of the smart wear device price are below 1000. Second, different background variables in the use motivation, target orientation and sports involvement have significant differences exist. Third, the use motivations will positively affect the target orientation; the use motivation among the curiosity and epidemics will positively affect the sports involvement; target orientation will positively affect sports involvement.
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