Background: As an epigenetic regulator, the transcriptional intermediary factor 1β (TIF1β)/ KAP1/TRIM28) has been linked to gene expression and chromatin remodeling at specific loci by association with members of the heterochromatin protein 1 (HP1) family and various other chromatin factors. The interaction between TIF1β and HP1 is crucial for heterochromatin formation and maintenance. The HP1-box, PXVXL, of TIF1β is responsible for its interaction with HP1. However, the underlying mechanism of how the interaction is regulated remains poorly understood.

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PurposeThis paper seeks to address a deficit in the criminal justice literature by examining patterns of electronic control device (ECD) use and effectiveness as reflected in 1,188 official police use‐of‐force report records collected over a three‐year period (2005‐2007) by the Washington State Patrol (WSP).Design/methodology/approachChi‐square, t‐test, ANOVA and logistic regression analysis were employed to analyze the data with respect to patterns of use and outcomes for officers and arrestees.FindingsThe findings observed indicate that the ECD tended to replace several other types of force used to gain compliance, tended to resolve incidents involving the use of force with fewer forms of force being used, and decreased officer injury rates. The ECD was rated as generally effective by officers, but not as effective as other methods of gaining compliance in life‐threatening situations. Results concerning suspect injury rates were somewhat mixed.Research limitations/implicationsRelying on the official self‐report from a single agency limits the ability to generalize to other law enforcement agencies.Practical implicationsThe paper indicates the importance of providing for the systematic collection of data on police use of force involving the threat of use and deployment of the ECD.Originality/valueA number of questions concerning ECD's appropriate use, effectiveness, and potential for harm have remained largely unanswered. The paper addresses this deficit in the criminal justice literature.

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Cryptococcus neoformans is a ubiquitously distributed human pathogen. It is also a model system for studying fungal virulence, physiology and differentiation. Light is known to inhibit sexual development via the evolutionarily conserved white collar proteins in C. neoformans. To dissect molecular mechanisms regulating this process, we have identified the SSN8 gene whose mutation suppresses the light-dependent CWC1 overexpression phenotype. Characterization of sex-related phenotypes revealed that Ssn8 functions as a negative regulator in both heterothallic a-α mating and same-sex mating processes. In addition, Ssn8 is involved in the suppression of other physiological processes including invasive growth, and production of capsule and melanin. Interestingly, Ssn8 is also required for the maintenance of cell wall integrity and virulence. Our gene expression studies confirmed that deletion of SSN8 results in de-repression of genes involved in sexual development and melanization. Epistatic and yeast two hybrid studies suggest that C. neoformans Ssn8 plays critical roles downstream of the Cpk1 MAPK cascade and Ste12 and possibly resides at one of the major branches downstream of the Cwc complex in the light-mediated sexual development pathway. Taken together, our studies demonstrate that the conserved Mediator protein Ssn8 functions as a global regulator which negatively regulates diverse physiological and developmental processes and is required for virulence in C. neoformans.