Xylella fastidiosa subsp. pauca is a xylem-limited bacterial phytopathogen currently found associated on many hectares with the “olive quick decline syndrome” in the Apulia region (Southern Italy), and the cultivars Ogliarola salentina and Cellina di Nardò result in being particularly sensitive to the disease. In order to find compounds showing the capability of reducing the population cell density of the pathogen within the leaves, we tested, in some olive orchards naturally-infected by the bacterium, a zinc-copper-citric acid biocomplex, namely Dentamet®, by spraying it to the crown, once per month, during spring and summer. The occurrence of the pathogen in the four olive orchards chosen for the trial was molecularly assessed. A 1H NMR metabolomic approach, in conjunction with a multivariate statistical analysis, was applied to investigate the metabolic pattern of both infected and treated adult olive cultivars, Ogliarola salentina and Cellina di Nardò trees, in two sampling periods, performed during the first year of the trial. For both cultivars and sampling periods, the orthogonal partial least squares discriminant analysis (OPLS-DA) gave good models of separation according to the treatment application. In both cultivars, some metabolites such as quinic acid, the aldehydic form of oleoeuropein, ligstroside and phenolic compounds, were consistently found as discriminative for the untreated olive trees in comparison with the Dentamet®-treated trees. Quinic acid, a precursor of lignin, was confirmed as a disease biomarker for the olive trees infected by X. fastidiosa subsp. pauca. When treated with Dentamet®, the two cultivars showed a distinct response. A consistent increase in malic acid was observed for the Ogliarola salentina trees, whereas in the Cellina di Nardò trees the treatments attenuate the metabolic response to the infection. To note that in Cellina di Nardò trees at the first sampling, an increase in γ-aminobutyric acid (GABA) was observed. This study highlights how the infection incited by X. fastidiosa subsp. pauca strongly modifies the overall metabolism of olive trees, and how a zinc-copper-citric acid biocomplex can induce an early re-programming of the metabolic pathways in the infected trees.
Background: Xylella fastidiosa is a Gram-negative bacterium which lives in the xylem of plants, causing its occlusion and other alterations inducing eventually the death of the infected plants. In Salento, the sub-peninsula in the south-eastern of Apulia Region (southern Italy), the infection of X. fastidiosa has been associated with the widespread presence of CoDiRO (complex of parasitic agents that constitute the so-called "olive quick decline syndrome") and currently represents a serious local emergence. The need to adopt specific agronomic measures to contrast the further disease spread has been recently raised. The extensive NMR-based metabolomic approach to study the metabolic effects of CoDiRO on local olive cultivars such as Ogliarola salentina and Cellina di Nardò was used. Results:In this study, the effects of a CE approved fertilizer containing zinc, copper, and citric acid, known as DENTAMET ® , on CoDiRO-exhibiting olive trees infected by X. fastidiosa were studied by 1 H NMR spectroscopy. The changes in the metabolomic profiles of aqueous extracts obtained from leaves of the two olive cultivars are reported. Upon the DENTAMET ® treatments, different and opposite polyphenolic and sugars patterns in the two cultivars, which showed a different incidence and severity of disease before the treatments, were detected. Conclusions:Differences in the sugars and polyphenols content of treated versus untreated trees could potentially contribute to the syndrome monitoring and might be related to the X. fastidiosa presence.
In this work, we tested a NMR-based MVA method to assess the authenticity of EVOOs cultivar (from specific origin) declaration by using a total of over a 100 samples coming from different selected areas of Southern Italian Regions (Apulia and Calabria). We built a reference model, by performing unsupervised PCA and supervised PLS-DA, OPLS-DA analyses on the 1 HNMR spectra of a 40 monovarietal EVOOs training set. These included 10 Coratina (the most popular Apulian cultivar) together with 10 Picholine, 10 Ogliarola Barese, and 10 Carolea samples (the most common Coratina bitterness smoothers cultivars used in Coratina-based blends). The training set originated PLS-DA reference model was profitably used to assess the class membership for a series of declared monocultivar EVOO samples and to obtain a clear indication for the composition of Coratina-based blends. By using the same reference model we analyzed in blind a test set formed by 40 unknown samples coming from the same areas and we predicted their class or blend composition affinity (later confirmed by suppliers as Coratina-or Coratina-based blends). This proposed method could be a simple and quick tool to assess, also for trading purpose, the cultivar (from given geographical areas) declaration of labeled EVOOs.Practical applications: EVOO is one of the most important element of the Mediterranean diet with its numerous health benefits due to the chemical composition (unsaturated fatty acids and minor components). For this high commercial value in the recent years, EVOO has been processed to adulteration process with seeds oils, refined pomace, and esterified oils with serious damage to its quality. In order to guarantee the quality of this important product, EU Regulation 182 of March 6, 2009, stated the obligatory labeling of EVOO samples with the geographical production area for all European countries. Surprisingly, this regulation lacks of an official methodology to assess the origin and the cultivar of EVOOs. A quick and reproducible method to check the label declaration of commercial EVOOs could be attractive for industry and ensure transparency for consumer choices.
Nine hundred extra virgin olive oils (EVOO) were extracted from individual olive trees of four olive cultivars (Coratina, Cima di Mola, Ogliarola, Peranzana), originating from the provinces of Bari and Foggia (Apulia region, Southern Italy) and collected during two consecutive harvesting seasons (2013/14 and 2014/15). Following genetic identification of individual olive trees, a detailed Apulian EVOO NMR database was built using 900 oils samples obtained from 900 cultivar certified single trees. A study on the olive oil lipid profile was carried out by statistical multivariate analysis (Principal Component Analysis, PCA, Partial Least-Squares Discriminant Analysis, PLS-DA, Orthogonal Partial Least-Squares Discriminant Analysis, OPLS-DA). Influence of cultivar and weather conditions, such as the summer rainfall, on the oil metabolic profile have been evaluated. Mahalanobis distances and J2 criterion have been measured to assess the quality of resulting scores clusters for each cultivar in the two harvesting campaigns. The four studied cultivars showed non homogeneous behavior. Notwithstanding the geographical spread and the wide number of samples, Coratina showed a consistent behavior of its metabolic profile in the two considered harvests. Among the other three Peranzana showed the second more consistent behavior, while Cima di Mola and Ogliarola having the biggest change over the two years.
We report a study on the chemical characterization of 102 monovarietal micro‐extracted and genetically characterized extra virgin olive oils (EVOOs) from Coratina, Ogliarola Barese and Cima di Mola cultivar, according to “Terra di Bari” (Apulia, southeast Italy) PDO requirement. Three additional geographical mentions, all belonging to the same Bari district (Bitonto, Castel del Monte and Murgia dei Trulli e delle Grotte), were studied and potential microclimate differences were evaluated. Our results indicate the possibility of distinguishing EVOOs from the same “Terra di Bari” PDO with respect to different cultivars and (to some extent) different subareas. In particular, two cultivars (Ogliarola Barese and Coratina), obtained from the same Bitonto area, and a single cultivar (Coratina), obtained from Bitonto and Castel del Monte subareas, were compared to investigate the micro‐area pedoclimatic effect. Finally, 1H NMR data were found significant for classification purposes of unknown EVOO samples, the results of which were included in the model space, as they were correctly predicted by the OPLS‐DA obtained by 1H NMR data. This work aims to classify commercial “Terra di Bari” PDO EVOOs by comparison of the declared cultivar and geographical origin with a reference dataset of genetically characterized micro‐extracted monovarietal oils. In conclusion, the effect of the pedoclimatic micro‐areas of origin on the Coratina cultivar, which is the main component of the “Terra di Bari” PDO, has been studied in order to improve the traceability of the raw material used to produce this valuable food.
Jellyfish outbreaks in marine coastal areas represent an emergent problem worldwide, with negative consequences on human activities and ecosystem functioning. However, potential positive effects of jellyfish biomass proliferation may be envisaged as a natural source of bioactive compounds of pharmaceutical interest. We investigated the biochemical composition of mature female gonads and lysozyme antibacterial activity of oocytes in the Mediterranean barrel jellyfish Rhizostoma pulmo. Chemical characterization was performed by means of multinuclear and multidimensional NMR spectroscopy. The ovaries of R. pulmo were mainly composed of water (93.7 ± 1.9% of wet weight), with organic matter (OM) and dry weight made respectively of proteins (761.76 ± 25.11 µg mg−1 and 45.7 ± 1.5%), lipids (192.17 ± 10.56 µg mg−1 and 9.6 ± 0.6%), and carbohydrates (59.66 ± 2.72 µg mg−1 and 3.7 ± 0.3%). The aqueous extract of R. pulmo gonads contained free amino acids, organic acids, and derivatives; the lipid extract was composed of triglycerides (TG), polyunsaturated fatty acids (PUFAs), diunsaturated fatty acids (DUFAs), monounsaturated fatty acids (MUFAs), saturated fatty acids (SFAs), and minor components such as sterols and phospholipids. The R. pulmo oocyte lysate exhibited an antibacterial lysozyme-like activity (mean diameter of lysis of 9.33 ± 0.32 mm corresponding to 1.21 mg/mL of hen egg-white lysozyme). The occurrence of defense molecules is a crucial mechanism to grant healthy development of mature eggs and fertilized embryos (and the reproductive success of the species) by preventing marine bacterial overgrowth. As a corollary, these results call for future investigations for an exploitation of R. pulmo biomasses as a resource of bioactive metabolites of biotechnological importance including pharmaceuticals and nutrition.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.