The TOCA family of F-BAR–containing proteins bind to and remodel lipid bilayers via their conserved F-BAR domains, and regulate actin dynamics via their N-Wasp binding SH3 domains. Thus, these proteins are predicted to play a pivotal role in coordinating membrane traffic with actin dynamics during cell migration and tissue morphogenesis. By combining genetic analysis in Caenorhabditis elegans with cellular biochemical experiments in mammalian cells, we showed that: i) loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. Oocyte endocytosis defects correlated well with reduced egg production in these mutants. ii) CeTOCA proteins localize to cell–cell junctions and are required for proper embryonic morphogenesis, to position hypodermal cells and to organize junctional actin and the junction-associated protein AJM-1. iii) Double mutant analysis indicated that the toca genes act in the same pathway as the nematode homologue of N-WASP/WASP, wsp-1. Furthermore, mammalian TOCA-1 and C. elegans CeTOCAs physically associated with N-WASP and WSP-1 directly, or WAVE2 indirectly via ABI-1. Thus, we propose that TOCA proteins control tissues morphogenesis by coordinating Clathrin-dependent membrane trafficking with WAVE and N-WASP–dependent actin-dynamics.

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This paper illustrates a land consumption map for Italy (year 2017) at a scale 1:1,300,000, and the assessment of its changes (2012-2017). We define land consumption as the replacement of a non-artificial land cover to an artificial land cover, both permanent and no-permanent. The maps are a 10 m spatial resolution raster, produced by photointerpretation of very high resolution images and semiautomatic classification of high resolution remote sensing images. An overall accuracy of 97.7% for the map of 2012 and of 99.66% for the map of 2017 was obtained. The results suggest that the method proposed is appropriate to detect land consumption, both for the urban densification and for the sprawling phenomena, from national to local level. Furthermore, because of the high spatial resolution and the classification scheme adopted, it is suitable for an effective monitoring system, compared to other existing classification systems or monitoring programs.

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